Signal Transduction Antibodies 1
Anti-ATP6V1E2 Antibody (CAB12204)
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- SKU:
- CAB12204
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Description
| Antibody Name: | Anti-ATP6V1E2 Antibody |
| Antibody SKU: | CAB12204 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 90-180 of human ATP6V1E2 (NP_542384.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, Mouse brain, Mouse liver, Rat brain, Rat liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 90-180 of human ATP6V1E2 (NP_542384.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | LISD LLSE AKLR LSRI VEDP EVYQ GLLD KLVL QGLL RLLE PVMI VRCR PQDL LLVE AAVQ KAIP EYMT ISQK HVEV QIDK EAYL AVNA AGG |
| Gene ID: | 90423 |
| Uniprot: | Q96A05 |
| Cellular Location: | |
| Calculated MW: | 26kDa |
| Observed MW: | 35kDa |
| Synonyms: | ATP6V1E2, ATP6E1, ATP6EL2, ATP6V1EL2, VMA4 |
| Background: |
| UniProt Protein Function: | ATP6V1E2: Subunit of the peripheral V1 complex of vacuolar ATPase essential for assembly or catalytic function. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. This isoform is essential for energy coupling involved in acidification of acrosome. Belongs to the V-ATPase E subunit family. |
| UniProt Protein Details: | Protein type:EC 3.6.3.14; Energy Metabolism - oxidative phosphorylation; Hydrolase Chromosomal Location of Human Ortholog: 2p21 Cellular Component: cytosol Molecular Function:protein binding Biological Process: insulin receptor signaling pathway; transferrin transport |
| UniProt Code: | Q96A05 |
| NCBI GenInfo Identifier: | 74731076 |
| NCBI Gene ID: | 90423 |
| NCBI Accession: | Q96A05.1 |
| UniProt Related Accession: | Q96A05 |
| Molecular Weight: | 26kDa |
| NCBI Full Name: | V-type proton ATPase subunit E 2 |
| NCBI Synonym Full Names: | ATPase H+ transporting V1 subunit E2 |
| NCBI Official Symbol: | ATP6V1E2 |
| NCBI Official Synonym Symbols: | VMA4; ATP6E1; ATP6EL2; ATP6V1EL2 |
| NCBI Protein Information: | V-type proton ATPase subunit E 2 |
| UniProt Protein Name: | V-type proton ATPase subunit E 2 |
| UniProt Synonym Protein Names: | Vacuolar proton pump subunit E 2 |
| UniProt Gene Name: | ATP6V1E2 |
 | Western blot analysis of extracts of various cell lines, using ATP6V1E2 antibody (CAB12204) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
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