Signal Transduction Antibodies 1

Anti-ATP6V1A Antibody (CAB14706)

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SKU:
CAB14706
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction

Description

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Antibody Name:Anti-ATP6V1A Antibody
Antibody SKU:CAB14706
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 500-600 of human ATP6V1A (NP_001681.2).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:100 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:293T, LO2, HeLa, Mouse brain, Mouse heart, Mouse kidney
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 500-600 of human ATP6V1A (NP_001681.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:SLAE TDKI TLEV AKLI KDDF LQQN GYTP YDRF CPFY KTVG MLSN MIAF YDMA RRAV ETTA QSDN KITW SIIR EHMG DILY KLSS MKFK DPLK DGEA KIKS D
Gene ID:523
Uniprot:P38606
Cellular Location:
Calculated MW:64kDa/68kDa
Observed MW:68kDa
Synonyms:ATP6V1A, ATP6A1, ATP6V1A1, HO68, VA68, VPP2, Vma1, ARCL2D
Background:This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is one of two V1 domain A subunit isoforms and is found in all tissues. Transcript variants derived from alternative polyadenylation exist.
UniProt Protein Function:ATP6V1A: Catalytic subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. Belongs to the ATPase alpha/beta chains family.
UniProt Protein Details:

Protein type:EC 3.6.3.14; Energy Metabolism - oxidative phosphorylation; Hydrolase

Chromosomal Location of Human Ortholog: 3q13.31

Cellular Component: microvillus; mitochondrion; integral to plasma membrane; lysosomal membrane; apical plasma membrane; plasma membrane; proton-transporting two-sector ATPase complex; cytosol

Molecular Function:hydrogen ion transporting ATPase activity, rotational mechanism; ATP binding

Biological Process: ATP metabolic process; interaction with host; cellular iron ion homeostasis; transport; ATP hydrolysis coupled proton transport; insulin receptor signaling pathway; transferrin transport; transmembrane transport

NCBI Summary:This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is one of two V1 domain A subunit isoforms and is found in all tissues. Transcript variants derived from alternative polyadenylation exist. [provided by RefSeq, Jul 2008]
UniProt Code:P38606
NCBI GenInfo Identifier:22096378
NCBI Gene ID:523
NCBI Accession:P38606.2
UniProt Related Accession:P38606
Molecular Weight:
NCBI Full Name:V-type proton ATPase catalytic subunit A
NCBI Synonym Full Names:ATPase H+ transporting V1 subunit A
NCBI Official Symbol:ATP6V1A  
NCBI Official Synonym Symbols:HO68; VA68; VPP2; Vma1; ARCL2D; ATP6A1; IECEE3; ATP6V1A1  
NCBI Protein Information:V-type proton ATPase catalytic subunit A
UniProt Protein Name:V-type proton ATPase catalytic subunit A
UniProt Synonym Protein Names:V-ATPase 69 kDa subunit; Vacuolar ATPase isoform VA68; Vacuolar proton pump subunit alpha
UniProt Gene Name:ATP6V1A  
UniProt Entry Name:VATA_HUMAN
Anti-ATP6V1A Antibody (CAB14706)Western blot analysis of extracts of various cell lines, using ATP6V1A antibody (CAB14706) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 3min.
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