Antibody Name:	Anti-ATP6AP2 Antibody
Antibody SKU:	CAB6531
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 251-350 of human ATP6AP2 (NP_005756.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	SW480, Mouse kidney, Mouse brain, Mouse eye, Rat brain, Rat kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 251-350 of human ATP6AP2 (NP_005756.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MYSL YGGN AVVE LVTV KSFD TSLI RKTR TILE AKQA KNPA SPYN LAYK YNFE YSVV FNMV LWIM IALA LAVI ITSY NIWN MDPG YDSI IYRM TNQK IRMD
Gene ID:	10159
Uniprot:	O75787
Cellular Location:	Membrane, Single-pass type I membrane protein
Calculated MW:	35kDa/39kDa
Observed MW:	48kDa

Synonyms:

ATP6AP2, APT6M8-9, ATP6IP2, ATP6M8-9, ELDF10, HT028, M8-9, MRXE, MRXSH, MSTP009, PRR, RENR, XMRE, XPDS

Background:

This gene encodes a protein that is associated with adenosine triphosphatases (ATPases). Proton-translocating ATPases have fundamental roles in energy conservation, secondary active transport, acidification of intracellular compartments, and cellular pH homeostasis. There are three classes of ATPases- F, P, and V. The vacuolar (V-type) ATPases have a transmembrane proton-conducting sector and an extramembrane catalytic sector. The encoded protein has been found associated with the transmembrane sector of the V-type ATPases.

UniProt Protein Function:	ATP6AP2: Functions as a renin and prorenin cellular receptor. May mediate renin-dependent cellular responses by activating ERK1 and ERK2. By increasing the catalytic efficiency of renin in AGT/angiotensinogen conversion to angiotensin I, it may also play a role in the renin-angiotensin system (RAS). Defects in ATP6AP2 are a cause of mental retardation X- linked with epilepsy (MRXE). MRXE is a syndromic mental retardation. Patients manifest mild to moderate mental retardation associated with epilepsy, delays in motor milestones and speech acquisition in infancy.
UniProt Protein Details:	Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: Xp11.4 Cellular Component: neuron projection; integral to membrane; plasma membrane; external side of plasma membrane Molecular Function:protein binding; enzyme binding; receptor activity; aspartic-type endopeptidase activity Biological Process: positive regulation of transforming growth factor-beta1 production; rostrocaudal neural tube patterning; cellular protein metabolic process; regulation of MAPKKK cascade; proteolysis; angiotensin maturation; positive regulation of Wnt receptor signaling pathway; eye pigmentation Disease: Mental Retardation, X-linked, Syndromic, Hedera Type; Parkinsonism With Spasticity, X-linked
NCBI Summary:	This gene encodes a protein that is associated with adenosine triphosphatases (ATPases). Proton-translocating ATPases have fundamental roles in energy conservation, secondary active transport, acidification of intracellular compartments, and cellular pH homeostasis. There are three classes of ATPases- F, P, and V. The vacuolar (V-type) ATPases have a transmembrane proton-conducting sector and an extramembrane catalytic sector. The encoded protein has been found associated with the transmembrane sector of the V-type ATPases. [provided by RefSeq, Jul 2008]
UniProt Code:	O75787
NCBI GenInfo Identifier:	66774158
NCBI Gene ID:	10159
NCBI Accession:	O75787.2
UniProt Secondary Accession:	O75787,Q5QTQ7, Q6T7F5, Q8NBP3, Q8NG15, Q96FV6, Q96LB5 Q9H2P8, Q9UG89, B7Z9I3,
UniProt Related Accession:	O75787
Molecular Weight:	350
NCBI Full Name:	Renin receptor
NCBI Synonym Full Names:	ATPase, H+ transporting, lysosomal accessory protein 2
NCBI Official Symbol:	ATP6AP2
NCBI Official Synonym Symbols:	PRR; M8-9; MRXE; RENR; XMRE; XPDS; HT028; MRXSH; ELDF10; ATP6IP2; MSTP009; APT6M8-9; ATP6M8-9
NCBI Protein Information:	renin receptor; N14F; prorenin receptor; V-ATPase M8.9 subunit; renin/prorenin receptor; ER-localized type I transmembrane adaptor; embryonic liver differentiation factor 10; ATPase H(+)-transporting lysosomal-interacting protein 2; ATPase, H+ transporting, lysosomal interacting protein 2; vacuolar ATP synthase membrane sector-associated protein M8-9; vacuolar proton ATP synthase membrane sector associated protein M8-9; ATPase, H+ transporting, lysosomal (vacuolar proton pump) membrane sector associated protein M8-9
UniProt Protein Name:	Renin receptor
UniProt Synonym Protein Names:	ATPase H(+)-transporting lysosomal accessory protein 2; ATPase H(+)-transporting lysosomal-interacting protein 2; ER-localized type I transmembrane adaptor; Embryonic liver differentiation factor 10; N14F; Renin/prorenin receptor; Vacuolar ATP synthase membrane sector-associated protein M8-9; ATP6M8-9; V-ATPase M8.9 subunit
Protein Family:	Renin receptor
UniProt Gene Name:	ATP6AP2
UniProt Entry Name:	RENR_HUMAN

	Western blot analysis of extracts of various cell lines, using ATP6AP2 antibody (CAB6531) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunofluorescence analysis of HeLa cells using ATP6AP2 antibody (CAB6531). Blue: DAPI for nuclear staining.