Signal Transduction Antibodies 1
Anti-ATP6AP1 Antibody (CAB1209)
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- SKU:
- CAB1209
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Description
| Antibody Name: | Anti-ATP6AP1 Antibody |
| Antibody SKU: | CAB1209 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 90-390 of human ATP6AP1 (NP_001174.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | THP-1, OVCAR3, PC-3, Mouse brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 90-390 of human ATP6AP1 (NP_001174.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | LLFL QDKL SIED FTAY GGVF GNKQ DSAF SNLE NALD LAPS SLVL PAVD WYAV STLT TYLQ EKLG ASPL HVDL ATLR ELKL NASL PALL LIRL PYTA SSGL MAPR EVLT GNDE VIGQ VLST LKSE DVPY TAAL TAVR PSRV ARDV AVVA GGLG RQLL QKQP VSPV IHPP VSYN DTAP RILF WAQN FSVA YKDQ WEDL TPLT FGVQ ELNL TGSF WNDS FARL SLTY ERLF GTTV TFKF ILAN RLYP VSAR HWFT MERL EVHS NGSV AYFN ASQV TGPS IYSF HCEY VSSL SKKG SLLV ARTQ P |
| Gene ID: | 537 |
| Uniprot: | Q15904 |
| Cellular Location: | Single-pass membrane protein, Vacuole membrane |
| Calculated MW: | 52kDa |
| Observed MW: | 50kDa |
| Synonyms: | ATP6AP1, 16A, ATP6IP1, ATP6S1, Ac45, CF2, VATPS1, XAP-3, XAP3 |
| Background: | This gene encodes a component of a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. Vacuolar ATPase (V-ATPase) is comprised of a cytosolic V1 (site of the ATP catalytic site) and a transmembrane V0 domain. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, and receptor-mediated endocytosis. The encoded protein of this gene may assist in the V-ATPase-mediated acidification of neuroendocrine secretory granules. This protein may also play a role in early development. |
| UniProt Protein Function: | ATP6AP1: Vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. Belongs to the vacuolar ATPase subunit S1 family. |
| UniProt Protein Details: | Protein type:Transporter; EC 3.6.3.14; Energy Metabolism - oxidative phosphorylation; Hydrolase; Membrane protein, integral Chromosomal Location of Human Ortholog: Xq28 Cellular Component: vacuolar membrane; integral to membrane; endosome membrane; proton-transporting two-sector ATPase complex Molecular Function:transporter activity; hydrogen ion transporting ATP synthase activity, rotational mechanism; hydrogen ion transporting ATPase activity, rotational mechanism; Rab GTPase binding; ATP binding Biological Process: positive regulation of osteoblast differentiation; proton transport; cellular iron ion homeostasis; ATP hydrolysis coupled proton transport; pH reduction; insulin receptor signaling pathway; positive regulation of bone resorption; transferrin transport; transmembrane transport; establishment of organelle localization; positive regulation of exocytosis |
| NCBI Summary: | This gene encodes a component of a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. Vacuolar ATPase (V-ATPase) is comprised of a cytosolic V1 (site of the ATP catalytic site) and a transmembrane V0 domain. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, and receptor-mediated endocytosis. The encoded protein of this gene may assist in the V-ATPase-mediated acidification of neuroendocrine secretory granules. This protein may also play a role in early development. [provided by RefSeq, Aug 2013] |
| UniProt Code: | Q15904 |
| NCBI GenInfo Identifier: | 12230759 |
| NCBI Gene ID: | 537 |
| NCBI Accession: | Q15904.2 |
| UniProt Secondary Accession: | Q15904,Q8NBT4, Q9H0C7, A6ZKI4, |
| UniProt Related Accession: | Q15904 |
| Molecular Weight: | 470 |
| NCBI Full Name: | V-type proton ATPase subunit S1 |
| NCBI Synonym Full Names: | ATPase, H+ transporting, lysosomal accessory protein 1 |
| NCBI Official Symbol: | ATP6AP1 |
| NCBI Official Synonym Symbols: | 16A; CF2; Ac45; XAP3; XAP-3; ATP6S1; VATPS1; ATP6IP1 |
| NCBI Protein Information: | V-type proton ATPase subunit S1; protein XAP-3; H-ATPase subunit; V-ATPase subunit S1; V-ATPase Ac45 subunit; V-ATPase S1 accessory protein; vacuolar proton pump subunit S1; ATPase, H+ transporting, lysosomal interacting protein 1; ATPase, H+ transporting, lysosomal (vacuolar proton pump), subunit 1 |
| UniProt Protein Name: | V-type proton ATPase subunit S1 |
| UniProt Synonym Protein Names: | Protein XAP-3; V-ATPase Ac45 subunit; V-ATPase S1 accessory protein; Vacuolar proton pump subunit S1 |
| Protein Family: | V-type proton ATPase |
| UniProt Gene Name: | ATP6AP1 |
| UniProt Entry Name: | VAS1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ATP6AP1 antibody (CAB1209) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
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