Cell Biology Antibodies 12
Anti-ATP6 Antibody (CAB8193)
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- SKU:
- CAB8193
- Product Type:
- Antibody
- Applications:
- WB
- Applications:
- IHC
- Applications:
- IF
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | ATP6 Rabbit Polyclonal Antibody |
| Antibody SKU: | CAB8193 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 100 to the C-terminus of mouse ATP6 (NP_904333.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:100 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | NCI-H460, SKOV3, MCF7, HeLa, Mouse brain, Mouse skeletal muscle |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 100 to the C-terminus of mouse ATP6 (NP_904333.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MNLS MAIP LWAG AVIT GFRH KLKS SLAH FLPQ GTPI SLIP MLII IETI SLFI QPMA LAVR LTAN ITAG HLLM HLIG GATL VLMN ISPP TATI TFII LLLL TILE FAVA LIQA YVFT LLVS LYLH DNT |
| Gene ID: | 17705 |
| Uniprot: | P00848 |
| Cellular Location: | Mitochondrion inner membrane, Multi-pass membrane protein |
| Calculated MW: | 25kDa |
| Observed MW: | 20kDa |
| Synonyms: | MT-ATP6, ATPase6, MTATP6, ATP6 |
| Background: |
| UniProt Protein Function: | ATP6: Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Key component of the proton channel; it may play a direct role in the translocation of protons across the membrane. Defects in MT-ATP6 are the cause of neurogenic muscle weakness, ataxia, and retinitis pigmentosa (NARP). Defects in MT-ATP6 are a cause of Leber hereditary optic neuropathy (LHON). LHON is a maternally inherited disease resulting in acute or subacute loss of central vision, due to optic nerve dysfunction. Cardiac conduction defects and neurological defects have also been described in some patients. LHON results from primary mitochondrial DNA mutations affecting the respiratory chain complexes. Defects in MT-ATP6 are a cause of Leigh syndrome (LS). LS is a severe neurological disorder characterized by bilaterally symmetrical necrotic lesions in subcortical brain regions. Defects in MT-ATP6 are a cause of mitochondrial infantile bilateral striatal necrosis (MIBSN). Bilateral striatal necrosis is a neurological disorder resembling Leigh syndrome. Belongs to the ATPase A chain family.Protein type: Transporter, ion channel; Membrane protein, multi-pass; Transporter; Membrane protein, integral; MitochondrialCellular Component: mitochondrion; membrane; proton-transporting ATP synthase complex, coupling factor F(o); mitochondrial inner membrane; integral to membraneMolecular Function: hydrogen ion transmembrane transporter activityBiological Process: ATP synthesis coupled proton transport; proton transport; transport; ATP biosynthetic process; ion transport |
| UniProt Protein Details: | |
| NCBI Summary: | |
| UniProt Code: | P00848 |
| NCBI GenInfo Identifier: | 34538603 |
| NCBI Gene ID: | |
| NCBI Accession: | NP_904333.1 |
| UniProt Secondary Accession: | P00848 |
| UniProt Related Accession: | P00848 |
| Molecular Weight: | ~ 25kDa |
| NCBI Full Name: | ATP synthase F0 subunit 6 (mitochondrion) |
| NCBI Synonym Full Names: | |
| NCBI Official Symbol: | |
| NCBI Official Synonym Symbols: | |
| NCBI Protein Information: | |
| UniProt Protein Name: | ATP synthase subunit a |
| UniProt Synonym Protein Names: | F-ATPase protein 6 |
| Protein Family: | |
| UniProt Gene Name: | Mtatp6 |
| UniProt Entry Name: | ATP6_MOUSE |
 | Western blot analysis of extracts of various cell lines, using ATP6 antibody (CAB8193) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 10s. |
 | Immunofluorescence analysis of U2OS cells using ATP6 antibody (CAB8193) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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