Cell Biology Antibodies 14
Anti-ATP5I Antibody (CAB16769)
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- SKU:
- CAB16769
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-ATP5I Antibody |
| Antibody SKU: | CAB16769 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-69 of human ATP5I (NP_009031.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-87MG, LO2, 293T, HeLa |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-69 of human ATP5I (NP_009031.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MVPP VQVS PLIK LGRY SALF LGVA YGAT RYNY LKPR AEEE RRIA AEEK KKQD ELKR IARE LAED DSIL K |
| Gene ID: | 521 |
| Uniprot: | P56385 |
| Cellular Location: | Mitochondrion, Mitochondrion inner membrane |
| Calculated MW: | 7kDa |
| Observed MW: | Refer to figures |
 | Western blot - ATP5I Rabbit pAb (CAB16769) |
| Synonyms: | ATP5I, ATP5K |
| Background: | Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the e subunit of the Fo complex. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | ATP5I: Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain. Minor subunit located with subunit a in the membrane. Belongs to the ATPase e subunit family. |
| UniProt Protein Details: | Protein type:EC 3.6.3.14; Energy Metabolism - oxidative phosphorylation; Mitochondrial Chromosomal Location of Human Ortholog: 4p16.3 Cellular Component: mitochondrial inner membrane; mitochondrial proton-transporting ATP synthase complex; mitochondrion Molecular Function:ATPase activity; hydrogen ion transmembrane transporter activity; transmembrane transporter activity Biological Process: cellular metabolic process; mitochondrial ATP synthesis coupled proton transport |
| NCBI Summary: | Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the e subunit of the Fo complex. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jun 2010] |
| UniProt Code: | P56385 |
| NCBI GenInfo Identifier: | 3023369 |
| NCBI Gene ID: | 521 |
| NCBI Accession: | P56385.2 |
| UniProt Secondary Accession: | P56385,Q0D2L9, |
| UniProt Related Accession: | P56385 |
| Molecular Weight: | 7,933 Da |
| NCBI Full Name: | ATP synthase subunit e, mitochondrial |
| NCBI Synonym Full Names: | ATP synthase, H+ transporting, mitochondrial Fo complex subunit E |
| NCBI Official Symbol: | ATP5I |
| NCBI Official Synonym Symbols: | ATP5K |
| NCBI Protein Information: | ATP synthase subunit e, mitochondrial |
| UniProt Protein Name: | ATP synthase subunit e, mitochondrial |
| Protein Family: | ATP synthase |
| UniProt Gene Name: | ATP5I |
| UniProt Entry Name: | ATP5I_HUMAN |
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