Metabolism Antibodies 3

Anti-ATP5A1 Antibody (CAB11217)

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SKU:
CAB11217
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Monoclonal Antibody
Research Area:
Metabolism

Description

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Antibody Name:Anti-ATP5A1 Antibody
Antibody SKU:CAB11217
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthesized peptide derived from human ATP5A1
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:293T, A-549, Mouse liver, Mouse heart, Rat brain, Rat heart
Immunogen:A synthesized peptide derived from human ATP5A1
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:498
Uniprot:P25705
Cellular Location:
Calculated MW:55kDa
Observed MW:55KDa
Anti-ATP5A1 AntibodyWestern blot - ATP5A1 Rabbit mAb (CAB11217)
Synonyms:ATP5A, ATP5AL2, ATPM, COXPD22, HEL-S-123m, MC5DN4, MOM2, OMR, ORM, hATP1
Background:This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, using an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the alpha subunit of the catalytic core. Alternatively spliced transcript variants encoding the different isoforms have been identified. Pseudogenes of this gene are located on chromosomes 9, 2, and 16. [provided by RefSeq, Mar 2012]
UniProt Protein Function:ATP5A1: Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Subunit alpha does not bear the catalytic high-affinity ATP-binding sites. Belongs to the ATPase alpha/beta chains family.
UniProt Protein Details:

Protein type:EC 3.6.3.14; Transporter; Mitochondrial; Hydrolase; Membrane protein, integral; Energy Metabolism - oxidative phosphorylation

Chromosomal Location of Human Ortholog: 18q21

Cellular Component: membrane; mitochondrial inner membrane; mitochondrial matrix; mitochondrial proton-transporting ATP synthase complex; mitochondrion; plasma membrane; signalosome

Molecular Function:ATP binding; ATPase activity; hydrogen ion transporting ATP synthase activity, rotational mechanism; MHC class I protein binding; protein binding; transmembrane transporter activity

Biological Process: ATP biosynthetic process; embryonic development; lipid metabolic process; mitochondrial ATP synthesis coupled proton transport; negative regulation of endothelial cell proliferation

Disease: Combined Oxidative Phosphorylation Deficiency 22; Mitochondrial Complex V (atp Synthase) Deficiency, Nuclear Type 4

NCBI Summary:This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, using an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the alpha subunit of the catalytic core. Alternatively spliced transcript variants encoding the different isoforms have been identified. Pseudogenes of this gene are located on chromosomes 9, 2, and 16. [provided by RefSeq, Mar 2012]
UniProt Code:P25705
NCBI GenInfo Identifier:114517
NCBI Gene ID:498
NCBI Accession:P25705.1
UniProt Secondary Accession:P25705,Q53XX6, Q8IXV2, Q96FB4, Q96HW2, Q96IR6, Q9BTV8 A8K092, B4DY56, K7ENP3,
UniProt Related Accession:P25705
Molecular Weight:57,547 Da
NCBI Full Name:ATP synthase subunit alpha, mitochondrial
NCBI Synonym Full Names:ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle
NCBI Official Symbol:ATP5A1  
NCBI Official Synonym Symbols:OMR; ORM; ATPM; MOM2; ATP5A; hATP1; MC5DN4; ATP5AL2; COXPD22; HEL-S-123m  
NCBI Protein Information:ATP synthase subunit alpha, mitochondrial
UniProt Protein Name:ATP synthase subunit alpha, mitochondrial
UniProt Gene Name:ATP5A1  
UniProt Entry Name:ATPA_HUMAN
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