Cell Biology Antibodies 1

Anti-ATP2B4 Antibody (CAB10105)

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SKU:
CAB10105
Product Type:
Antibody
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-ATP2B4 Antibody
Antibody SKU:CAB10105
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1141-1205 of human ATP2B4 (NP_001675.3).
Application:WB
Recommended Dilution:WB 1:1000 - 1:4000
Reactivity:Mouse
Positive Samples:Mouse brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1141-1205 of human ATP2B4 (NP_001675.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:ELPR TPLL DEEE EENP DKAS KFGT RVLL LDGE VTPY ANTN NNAV DCNQ VQLP QSDS SLQS LETS V
Gene ID:493
Uniprot:P23634
Cellular Location:Cell membrane, Cell projection, Multi-pass membrane protein, cilium, flagellum membrane
Calculated MW:124-137kDa
Observed MW:138kDa
Synonyms:ATP2B4, ATP2B2, MXRA1, PMCA4, PMCA4b, PMCA4x
Background:The protein encoded by this gene belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. This gene encodes the plasma membrane calcium ATPase isoform 4. Alternatively spliced transcript variants encoding different isoforms have been identified.
UniProt Protein Function:PMCA4: a plasma membrane calcium pump. A magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell. Eight splice-variant isoforms have been described.
UniProt Protein Details:

Protein type:Transporter; Transporter, ion channel; EC 3.6.3.8; Membrane protein, integral; Hydrolase; Channel, calcium; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 1q32.1

Cellular Component: caveola; integral to plasma membrane; intracellular membrane-bound organelle; membrane; plasma membrane; protein complex; T-tubule; Z disc

Molecular Function:calcium-transporting ATPase activity; calmodulin binding; nitric-oxide synthase binding; PDZ domain binding; protein binding; protein phosphatase 2B binding; sodium channel regulator activity

Biological Process: cellular calcium ion homeostasis; negative regulation of nitric oxide biosynthetic process; negative regulation of nitric-oxide synthase activity; positive regulation of peptidyl-serine phosphorylation; regulation of transcription from RNA polymerase II promoter; response to hydrostatic pressure; sperm motility

NCBI Summary:The protein encoded by this gene belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. This gene encodes the plasma membrane calcium ATPase isoform 4. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
UniProt Code:P23634
NCBI GenInfo Identifier:14286105
NCBI Gene ID:493
NCBI Accession:P23634.2
UniProt Secondary Accession:P23634,Q13450, Q13452, Q13455, Q16817, Q7Z3S1, B1APW5 B1APW6,
UniProt Related Accession:P23634
Molecular Weight:136,584 Da
NCBI Full Name:Plasma membrane calcium-transporting ATPase 4
NCBI Synonym Full Names:ATPase plasma membrane Ca2+ transporting 4
NCBI Official Symbol:ATP2B4  
NCBI Official Synonym Symbols:MXRA1; PMCA4; ATP2B2; PMCA4b; PMCA4x  
NCBI Protein Information:plasma membrane calcium-transporting ATPase 4
UniProt Protein Name:Plasma membrane calcium-transporting ATPase 4
UniProt Synonym Protein Names:Matrix-remodeling-associated protein 1; Plasma membrane calcium ATPase isoform 4; Plasma membrane calcium pump isoform 4
Protein Family:Plasma membrane calcium-transporting ATPase
UniProt Gene Name:ATP2B4  
UniProt Entry Name:AT2B4_HUMAN
Anti-ATP2B4 Antibody (CAB10105)Western blot analysis of extracts of mouse brain, using ATP2B4 antibody (CAB10105) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
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