Cell Biology Antibodies 3

Anti-ATP1B1 Antibody (CAB12403)

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SKU:
CAB12403
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-ATP1B1 Antibody
Antibody SKU:CAB12403
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 65-240 of human ATP1B1 (NP_001668.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:U-251MG, Mouse brain, Mouse liver, Mouse kidney, Rat kidney, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 65-240 of human ATP1B1 (NP_001668.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:KPTY QDRV APPG LTQI PQIQ KTEI SFRP NDPK SYEA YVLN IVRF LEKY KDSA QRDD MIFE DCGD VPSE PKER GDFN HERG ERKV CRFK LEWL GNCS GLND ETYG YKEG KPCI IIKL NRVL GFKP KPPK NESL ETYP VMKY NPNV LPVQ CTGK RDED KDKV GNVE YFGL GNSP GFPL
Gene ID:481
Uniprot:P05026
Cellular Location:Cell membrane, Single-pass type II membrane protein
Calculated MW:34kDa/35kDa
Observed MW:50kDa
Synonyms:ATP1B1, ATP1B
Background:The protein encoded by this gene belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. The glycoprotein subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes a beta 1 subunit. Alternatively spliced transcript variants encoding different isoforms have been described, but their biological validity is not known.
UniProt Protein Function:ATP1B1: This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. Belongs to the X(+)/potassium ATPases subunit beta family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Transporter; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q24

Cellular Component: apical plasma membrane; basolateral plasma membrane; caveola; intracellular; membrane; myelin sheath; plasma membrane; sarcolemma; sodium:potassium-exchanging ATPase complex

Molecular Function:ATP binding; ATPase activator activity; ATPase activity; ATPase binding; drug binding; potassium ion binding; protein binding; protein C-terminus binding; sodium ion binding; sodium:potassium-exchanging ATPase activity

Biological Process: ATP metabolic process; blood coagulation; cardiac muscle contraction; cell adhesion; cellular calcium ion homeostasis; cellular potassium ion homeostasis; cellular sodium ion homeostasis; establishment and/or maintenance of transmembrane electrochemical gradient; leukocyte migration; positive regulation of ATPase activity; positive regulation of defense response to virus by host; potassium ion import; protein stabilization; regulation of gene expression; response to hypoxia; transmembrane transport

Disease: Hypertension, Essential

NCBI Summary:The protein encoded by this gene belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. The glycoprotein subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes a beta 1 subunit. Alternatively spliced transcript variants encoding different isoforms have been described, but their biological validity is not known. [provided by RefSeq, Mar 2010]
UniProt Code:P05026
NCBI GenInfo Identifier:114392
NCBI Gene ID:481
NCBI Accession:P05026.1
UniProt Secondary Accession:P05026,Q5TGZ3,
UniProt Related Accession:P05026
Molecular Weight:34,893 Da
NCBI Full Name:Sodium/potassium-transporting ATPase subunit beta-1
NCBI Synonym Full Names:ATPase Na+/K+ transporting subunit beta 1
NCBI Official Symbol:ATP1B1  
NCBI Official Synonym Symbols:ATP1B  
NCBI Protein Information:sodium/potassium-transporting ATPase subunit beta-1
UniProt Protein Name:Sodium/potassium-transporting ATPase subunit beta-1
UniProt Synonym Protein Names:Sodium/potassium-dependent ATPase subunit beta-1
UniProt Gene Name:ATP1B1  
UniProt Entry Name:AT1B1_HUMAN
Anti-ATP1B1 Antibody (CAB12403)Western blot analysis of extracts of various cell lines, using ATP1B1 antibody (CAB12403) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Anti-ATP1B1 Antibody (CAB12403)Immunofluorescence analysis of C6 cells using ATP1B1 antibody (CAB12403) at dilution of 1:100. Blue: DAPI for nuclear staining.
Anti-ATP1B1 Antibody (CAB12403)Immunofluorescence analysis of NIH/3T3 cells using ATP1B1 antibody (CAB12403) at dilution of 1:100. Blue: DAPI for nuclear staining.
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