Signal Transduction Antibodies 2

Anti-ATP1A4 Antibody (CAB15256)

(No reviews yet) Write a Review
SKU:
CAB15256
Product Type:
Antibody
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-ATP1A4 Antibody
Antibody SKU:CAB15256
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-90 of human ATP1A4 (NP_653300.2).
Application:WB
Recommended Dilution:WB 1:200 - 1:2000
Reactivity:Mouse, Rat
Positive Samples:Mouse testis, Mouse brain, Mouse kidney, Rat testis, Rat brain, Rat kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-90 of human ATP1A4 (NP_653300.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MGLW GKKG TVAP HDQS PRRR PKKG LIKK KMVK REKQ KRNM EELK KEVV MDDH KLTL EELS TKYS VDLT KGHS HQRA KEIL TRGG PNTV TP
Gene ID:480
Uniprot:Q13733
Cellular Location:Cell membrane, Multi-pass membrane protein
Calculated MW:19kDa/114kDa
Observed MW:100kDa
Synonyms:ATP1A4, ATP1A1, ATP1AL2
Background:The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 4 subunit. Alternatively spliced transcript variants encoding different isoforms have been identified.
UniProt Protein Function:ATP1A4: This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients. Plays a role in sperm motility. Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 3.6.3.9; Hydrolase; Membrane protein, integral; Membrane protein, multi-pass; Motility/polarity/chemotaxis; Transporter; Transporter, ion channel

Chromosomal Location of Human Ortholog: 1q23.2

Cellular Component: integral component of plasma membrane; plasma membrane; sodium:potassium-exchanging ATPase complex

Molecular Function:ATP binding; metal ion binding; sodium:potassium-exchanging ATPase activity

Biological Process: ATP hydrolysis coupled proton transport; establishment or maintenance of transmembrane electrochemical gradient; fertilization; potassium ion transport; regulation of cellular pH; regulation of membrane potential; sodium ion transport; sperm motility; spermatogenesis

NCBI Summary:The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 4 subunit. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
UniProt Code:Q13733
NCBI GenInfo Identifier:153946397
NCBI Gene ID:480
NCBI Accession:NP_653300.2
UniProt Secondary Accession:Q13733,Q504T2, Q7Z4I9, Q8TBN8, Q8WXA7, Q8WXH7, Q8WY13
UniProt Related Accession:Q13733
Molecular Weight:19,609 Da
NCBI Full Name:sodium/potassium-transporting ATPase subunit alpha-4 isoform 1
NCBI Synonym Full Names:ATPase Na+/K+ transporting subunit alpha 4
NCBI Official Symbol:ATP1A4  
NCBI Official Synonym Symbols:ATP1A1; ATP1AL2  
NCBI Protein Information:sodium/potassium-transporting ATPase subunit alpha-4
UniProt Protein Name:Sodium/potassium-transporting ATPase subunit alpha-4
UniProt Synonym Protein Names:Sodium pump subunit alpha-4
Protein Family:Sodium/potassium-transporting ATPase
UniProt Gene Name:ATP1A4  
Anti-ATP1A4 Antibody (CAB15256)Western blot analysis of extracts of various cell lines, using ATP1A4 antibody (CAB15256) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1S.
View AllClose

0 Reviews

View AllClose

Related Products

ATP1A4 Monoclonal Antibody

ATP1A4 Monoclonal Antibody

OverviewProduct Name:ATP1A4 Monoclonal AntibodyProduct Code:CAB23196Reactivity:Human, Mouse, RatApplications:Western blotting, Im