Name: Anti-ATP12A Antibody (CAB14372)
Brand: Signal Transduction Antibodies 1
SKU: CAB14372
Availability: OutOfStock

Antibody Name:	Anti-ATP12A Antibody
Antibody SKU:	CAB14372
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 890-980 of human ATP12A (NP_001172014.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human
Positive Samples:	A-431, 293T

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 890-980 of human ATP12A (NP_001172014.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	YFTV YAQE GFLP RTLI NLRV EWEK DYVN DLKD SYGQ EWTR YQRE YLEW TGYT AFFV GILV QQIA DLII RKTR RNSI FQQG LFRN KVIW VGI
Gene ID:	479
Uniprot:	P54707
Cellular Location:	Membrane, Multi-pass membrane protein
Calculated MW:	115kDa/116kDa
Observed MW:	116KDa

Synonyms:

ATP12A, ATP1AL1, HK

Background:

The protein encoded by this gene belongs to the family of P-type cation transport ATPases. This gene encodes a catalytic subunit of the ouabain-sensitive H+/K+ -ATPase that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for potassium absorption in various tissues. Two transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	ATP12A: Catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. Responsible for potassium absorption in various tissues. Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Transporter; Energy Metabolism - oxidative phosphorylation; Transporter, ion channel; EC 3.6.3.10; Membrane protein, integral; Membrane protein, multi-pass; Hydrolase Chromosomal Location of Human Ortholog: 13q12.12\|13q12.1-q12.3 Cellular Component: hydrogen:potassium-exchanging ATPase complex; intracellular membrane-bound organelle; plasma membrane Molecular Function:hydrogen:potassium-exchanging ATPase activity; sodium:potassium-exchanging ATPase activity Biological Process: ATP hydrolysis coupled proton transport; cellular potassium ion homeostasis; cellular sodium ion homeostasis; potassium ion import
NCBI Summary:	The protein encoded by this gene belongs to the family of P-type cation transport ATPases. This gene encodes a catalytic subunit of the ouabain-sensitive H+/K+ -ATPase that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for potassium absorption in various tissues. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]
UniProt Code:	P54707
NCBI GenInfo Identifier:	212287925
NCBI Gene ID:	479
NCBI Accession:	P54707.3
UniProt Secondary Accession:	P54707,Q13816, Q13817, Q16734, Q5W035, Q8N5U2,
UniProt Related Accession:	P54707
Molecular Weight:	116,053 Da
NCBI Full Name:	Potassium-transporting ATPase alpha chain 2
NCBI Synonym Full Names:	ATPase H+/K+ transporting non-gastric alpha2 subunit
NCBI Official Symbol:	ATP12A
NCBI Official Synonym Symbols:	HK; #945; ATP1AL1
NCBI Protein Information:	potassium-transporting ATPase alpha chain 2
UniProt Protein Name:	Potassium-transporting ATPase alpha chain 2
UniProt Synonym Protein Names:	Non-gastric H(+)/K(+) ATPase subunit alpha; Proton pump
Protein Family:	Potassium-transporting ATPase
UniProt Gene Name:	ATP12A
UniProt Entry Name:	AT12A_HUMAN

Western blot analysis of extracts of various cell lines, using ATP12A antibody (CAB14372) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.

Antibodies

ATP12A Antibody (PACO01817)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)