Cell Biology Antibodies 6

Anti-ATP10A Antibody (CAB15873)

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SKU:
CAB15873
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-ATP10A Antibody
Antibody SKU:CAB15873
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 440-690 of human ATP10A (NP_077816.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:A-549, U-251MG, DU-145, OVCAR3, HT-29, A-431
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 440-690 of human ATP10A (NP_077816.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:RRCT VSGV EYSH DANA QRLA RYQE ADSE EEEV VPRG GSVS QRGS IGSH QSVR VVHR TQST KSHR RTGS RAEA KRAS MLSK HTAF SSPM EKDI TPDP KLLE KVSE CDKS LAVA RHQE HLLA HLSP ELSD VFDF FIAL TICN TVVV TSPD QPRT KVRV RFEL KSPV KTIE DFLR RFTP SCLT SGCS SIGS LAAN KSSH KLGS SFPS TPSS DGML LRLE ERLG QPTS AIAS NGYS SQAD NWAS ELAQ EQES ERE
Gene ID:57194
Uniprot:O60312
Cellular Location:Cell membrane, Endoplasmic reticulum membrane, Multi-pass membrane protein
Calculated MW:17kDa/167kDa
Observed MW:170kDa
Synonyms:ATP10A, ATP10C, ATPVA, ATPVC
Background:The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of aminophospholipid-transporting ATPases. The aminophospholipid translocases transport phosphatidylserine and phosphatidylethanolamine from one side of a bilayer to another. This gene is maternally expressed. It maps within the most common interval of deletion responsible for Angelman syndrome, also known as 'happy puppet syndrome'.
UniProt Code:O60312
NCBI GenInfo Identifier:14424433
NCBI Gene ID:57194
NCBI Accession:NP_077816.1
UniProt Secondary Accession:O60312,Q969I4,
UniProt Related Accession:O60312
Molecular Weight:167,688 Da
NCBI Full Name:probable phospholipid-transporting ATPase VA
NCBI Synonym Full Names:ATPase, class V, type 10A
NCBI Official Symbol:ATP10A  
NCBI Official Synonym Symbols:ATPVA; ATPVC; ATP10C  
NCBI Protein Information:probable phospholipid-transporting ATPase VA; ATPase, Class V, type 10C; aminophospholipid translocase VA; P4-ATPase flippase complex alpha subunit ATP10A; ATPase type IV, phospholipid transporting (P-type)
UniProt Protein Name:Probable phospholipid-transporting ATPase VA
UniProt Synonym Protein Names:ATPase class V type 10A; Aminophospholipid translocase VA; P4-ATPase flippase complex alpha subunit ATP10A
Protein Family:Probable phospholipid-transporting ATPase
UniProt Gene Name:ATP10A  
UniProt Entry Name:AT10A_HUMAN
Anti-ATP10A Antibody (CAB15873)Western blot analysis of extracts of various cell lines, using ATP10A antibody (CAB15873) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
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