Antibody Name:	Anti-ATL1 Antibody
Antibody SKU:	CAB5302
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human ATL1 (NP_056999.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	SH-SY5Y, LOVO, H460, 293T, U-87MG, U-251MG, Mouse brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human ATL1 (NP_056999.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAKN RRDR NSWG GFSE KTYE WSSE EEEP VKKA GPVQ VLIV KDDH SFEL DETA LNRI LLSE AVRD KEVV AVSV AGAF RKGK SFLM DFML RYMY NQES VDWV GDYN EPLT GFSW RGGS ERET TGIQ IWSE IFLI NKPD GKKV AVLL MDTQ GTFD SQST LRDS ATVF ALST MISS IQVY NLSQ NVQE DDLQ HLQL FTEY GRLA MEET FLKP FQSL IFLV RDWS FPYE FSYG ADGG AKFL EKRL KVSG NQHE ELQN VRKH IHSC FTNI SCFL LPHP GLKV ATNP
Gene ID:	51062
Uniprot:	Q8WXF7
Cellular Location:	Cell projection, Endoplasmic reticulum membrane, Golgi apparatus membrane, Multi-pass membrane protein, axon
Calculated MW:	63kDa
Observed MW:	64kDa

Synonyms:

ATL1, AD-FSP, FSP1, GBP3, HSN1D, SPG3, SPG3A, atlastin1

Background:

The protein encoded by this gene is a GTPase and a Golgi body transmembrane protein. The encoded protein can form a homotetramer and has been shown to interact with spastin and with mitogen-activated protein kinase kinase kinase kinase 4. This protein may be involved in axonal maintenance as evidenced by the fact that defects in this gene are a cause of spastic paraplegia type 3. Three transcript variants encoding two different isoforms have been found for this gene.

UniProt Protein Function:	atlastin: GTPase tethering membranes through formation of trans- homooligomer and mediating homotypic fusion of endoplasmic reticulum membranes. Functions in endoplasmic reticulum tubular network biogenesis. May also regulate Golgi biogenesis. May regulate axonal development. Defects in ATL1 are the cause of spastic paraplegia autosomal dominant type 3 (SPG3); also known as Strumpell-Lorrain syndrome. Spastic paraplegia is a degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Defects in ATL1 are the cause of hereditary sensory neuropathy type 1D (HSN1D). HSN1D is a disease characterized by adult-onset distal axonal sensory neuropathy leading to mutilating ulcerations as well as hyporeflexia. Some patients may show features suggesting upper neuron involvement. Belongs to the GBP family. Atlastin subfamily.
UniProt Protein Details:	Protein type:Membrane protein, integral; Membrane protein, multi-pass; Vesicle; EC 3.6.5.- Chromosomal Location of Human Ortholog: 14q22.1 Cellular Component: Golgi membrane; Golgi apparatus; endoplasmic reticulum membrane; axon; endoplasmic reticulum; integral to membrane; Golgi cis cisterna Molecular Function:GTPase activity; identical protein binding; protein binding; GTP binding Biological Process: endoplasmic reticulum organization and biogenesis; axonogenesis; metabolic process; protein homooligomerization Disease: Neuropathy, Hereditary Sensory, Type Id; Spastic Paraplegia 3, Autosomal Dominant
NCBI Summary:	The protein encoded by this gene is a GTPase and a Golgi body transmembrane protein. The encoded protein can form a homotetramer and has been shown to interact with spastin and with mitogen-activated protein kinase kinase kinase kinase 4. This protein may be involved in axonal maintenance as evidenced by the fact that defects in this gene are a cause of spastic paraplegia type 3. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	Q8WXF7
NCBI GenInfo Identifier:	37999727
NCBI Gene ID:	51062
NCBI Accession:	Q8WXF7.1
UniProt Secondary Accession:	Q8WXF7,O95890, Q69YH7, Q96FK0, A6NND5, A8K2C0, G5E9T1
UniProt Related Accession:	Q8WXF7
Molecular Weight:	63,055 Da
NCBI Full Name:	Atlastin-1
NCBI Synonym Full Names:	atlastin GTPase 1
NCBI Official Symbol:	ATL1
NCBI Official Synonym Symbols:	FSP1; GBP3; SPG3; HSN1D; SPG3A; AD-FSP; atlastin1
NCBI Protein Information:	atlastin-1; GBP-3; hGBP3; GTP-binding protein 3; guanylate-binding protein 3; spastic paraplegia 3 protein A; brain-specific GTP-binding protein; guanine nucleotide-binding protein 3
UniProt Protein Name:	Atlastin-1
UniProt Synonym Protein Names:	Brain-specific GTP-binding protein; GTP-binding protein 3; GBP-3; hGBP3; Guanine nucleotide-binding protein 3; Spastic paraplegia 3 protein A
Protein Family:	Atlastin
UniProt Gene Name:	ATL1
UniProt Entry Name:	ATLA1_HUMAN

	Western blot analysis of extracts of various cell lines, using ATL1 antibody (CAB5302) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s.
	Immunofluorescence analysis of A549 cells using ATL1 antibody (CAB5302). Blue: DAPI for nuclear staining.