Autophagy Antibodies
Anti-ATG9A Antibody (CAB5571)
- SKU:
- CAB5571
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Autophagy
Description
| Antibody Name: | Anti-ATG9A Antibody |
| Antibody SKU: | CAB5571 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 180-290 of human ATG9A (NP_076990.4). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:1000 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human |
| Positive Samples: | 293T |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 180-290 of human ATG9A (NP_076990.4). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | WQEV QARI VQTQ KEHQ ICIH KREL TELD IYHR ILRF QNYM VALV NKSL LPLR FRLP GLGE AVFF TRGL KYNF ELIL FWGP GSLF LNEW SLKA EYKR GGQR LELA QRLS NRI |
| Gene ID: | 79065 |
| Uniprot: | Q7Z3C6 |
| Cellular Location: | Cytoplasmic vesicle, Endoplasmic reticulum membrane, Golgi apparatus, Late endosome membrane, Multi-pass membrane protein, autophagosome membrane, trans-Golgi network membrane |
| Calculated MW: | 60kDa/87kDa/94kDa |
| Observed MW: | 100kDa |
| Synonyms: | ATG9A, APG9L1, MGD3208, mATG9 |
| Background: |
| UniProt Protein Function: | ATG9A: Plays a role in autophagy. Cycles between a juxta- nuclear trans-Golgi network compartment and late endosomes. Nutrient starvation induces accumulation on autophagosomes. Starvation-dependent trafficking requires ULK1, ATG13 and FAM48A. Interacts with FAM48A. Belongs to the ATG9 family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Membrane protein, multi-pass; Vesicle; Membrane protein, integral; Autophagy Chromosomal Location of Human Ortholog: 2q35 Cellular Component: endosome; late endosome; membrane; recycling endosome; trans-Golgi network Molecular Function:protein binding Biological Process: autophagic vacuole formation; mitochondrion degradation |
| UniProt Code: | Q7Z3C6 |
| NCBI GenInfo Identifier: | 296439428 |
| NCBI Gene ID: | 79065 |
| NCBI Accession: | Q7Z3C6.3 |
| UniProt Secondary Accession: | Q7Z3C6,Q3ZAQ6, Q6P0N7, Q7Z317, Q7Z320, Q8NDK6, Q8WU65 Q9BVL5, Q9H6L1, Q9HAG7, |
| UniProt Related Accession: | Q7Z3C6 |
| Molecular Weight: | 60,694 Da |
| NCBI Full Name: | Autophagy-related protein 9A |
| NCBI Synonym Full Names: | autophagy related 9A |
| NCBI Official Symbol: | ATG9A |
| NCBI Official Synonym Symbols: | mATG9; APG9L1; MGD3208 |
| NCBI Protein Information: | autophagy-related protein 9A |
| UniProt Protein Name: | Autophagy-related protein 9A |
| UniProt Synonym Protein Names: | APG9-like 1; mATG9 |
| Protein Family: | Autophagy-related protein |
| UniProt Gene Name: | ATG9A |
| UniProt Entry Name: | ATG9A_HUMAN |
 | Western blot analysis of extracts of 293T cells, using ATG9A antibody (CAB5571). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Immunofluorescence analysis of U2OS cells using ATG9A antibody (CAB5571) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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