Cell Death Antibodies 1

Anti-ATG5 Antibody (CAB11427)

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SKU:
CAB11427
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death

Description

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Antibody Name:Anti-ATG5 Antibody
Antibody SKU:CAB11427
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 28-275 of human ATG5 (NP_004840.1).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:293T, C6, NIH/3T3, K562
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 28-275 of human ATG5 (NP_004840.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:TERE AEPY YLLL PRVS YLTL VTDK VKKH FQKV MRQE DISE IWFE YEGT PLKW HYPI GLLF DLLA SSSA LPWN ITVH FKSF PEKD LLHC PSKD AIEA HFMS CMKE ADAL KHKS QVIN EMQK KDHK QLWM GLQN DRFD QFWA INRK LMEY PAEE NGFR YIPF RIYQ TTTE RPFI QKLF RPVA ADGQ LHTL GDLL KEVC PSAI DPED GEKK NQVM IHGI EPML ETPL QWLS EHLS YPDN FLHI SIIP QPTD
Gene ID:9474
Uniprot:Q9H1Y0
Cellular Location:Cytoplasm, Peripheral membrane protein, Preautophagosomal structure membrane
Calculated MW:22kDa/32kDa
Observed MW:50kDa
Synonyms:ATG5, APG5, APG5-LIKE, APG5L, ASP, hAPG5
Background:The protein encoded by this gene, in combination with autophagy protein 12, functions as an E1-like activating enzyme in a ubiquitin-like conjugating system. The encoded protein is involved in several cellular processes, including autophagic vesicle formation, mitochondrial quality control after oxidative damage, negative regulation of the innate antiviral immune response, lymphocyte development and proliferation, MHC II antigen presentation, adipocyte differentiation, and apoptosis. Several transcript variants encoding different protein isoforms have been found for this gene.
UniProt Code:Q9H1Y0
NCBI GenInfo Identifier:17366828
NCBI Gene ID:9474
NCBI Accession:Q9H1Y0.2
UniProt Secondary Accession:Q9H1Y0,O60875, Q5JVR2, Q68DI4, Q9H2B8, Q9HCZ7,
UniProt Related Accession:Q9H1Y0
Molecular Weight:275
NCBI Full Name:Autophagy protein 5
NCBI Synonym Full Names:autophagy related 5
NCBI Official Symbol:ATG5  
NCBI Official Synonym Symbols:ASP; APG5; APG5L; hAPG5; APG5-LIKE  
NCBI Protein Information:autophagy protein 5; apoptosis-specific protein; ATG5 autophagy related 5 homolog
UniProt Protein Name:Autophagy protein 5
UniProt Synonym Protein Names:APG5-like; Apoptosis-specific protein
Protein Family:Autophagy protein
UniProt Gene Name:ATG5  
UniProt Entry Name:ATG5_HUMAN
Anti-ATG5 Antibody (CAB11427)Western blot analysis of extracts of various cell lines, using ATG5 antibody (CAB11427) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
Anti-ATG5 Antibody (CAB11427)Immunohistochemistry of paraffin-embedded human liver using ATG5 antibody (CAB11427) at dilution of 1:100 (40x lens).
Anti-ATG5 Antibody (CAB11427)Immunohistochemistry of paraffin-embedded rat pancreas using ATG5 antibody (CAB11427) at dilution of 1:100 (40x lens).
Anti-ATG5 Antibody (CAB11427)Immunohistochemistry of paraffin-embedded mouse pancreas using ATG5 antibody (CAB11427) at dilution of 1:100 (40x lens).
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