Cell Death Antibodies 1

Anti-ATG5 Antibody (CAB0203)

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SKU:
CAB0203
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death

Description

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Antibody Name:Anti-ATG5 Antibody
Antibody SKU:CAB0203
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-275 of human ATG5 (NP_004840.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, NIH/3T3, U-87MG
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-275 of human ATG5 (NP_004840.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MTDD KDVL RDVW FGRI PTCF TLYQ DEIT EREA EPYY LLLP RVSY LTLV TDKV KKHF QKVM RQED ISEI WFEY EGTP LKWH YPIG LLFD LLAS SSAL PWNI TVHF KSFP EKDL LHCP SKDA IEAH FMSC MKEA DALK HKSQ VINE MQKK DHKQ LWMG LQND RFDQ FWAI NRKL MEYP AEEN GFRY IPFR IYQT TTER PFIQ KLFR PVAA DGQL HTLG DLLK EVCP SAID PEDG EKKN QVMI HGIE PMLE TPLQ WLSE HLSY PDNF LHIS IIPQ PTD
Gene ID:9474
Uniprot:Q9H1Y0
Cellular Location:Cytoplasm, Peripheral membrane protein, Preautophagosomal structure membrane
Calculated MW:22kDa/32kDa
Observed MW:55kDa
Synonyms:ATG5, APG5, APG5-LIKE, APG5L, ASP, hAPG5
Background:The protein encoded by this gene, in combination with autophagy protein 12, functions as an E1-like activating enzyme in a ubiquitin-like conjugating system. The encoded protein is involved in several cellular processes, including autophagic vesicle formation, mitochondrial quality control after oxidative damage, negative regulation of the innate antiviral immune response, lymphocyte development and proliferation, MHC II antigen presentation, adipocyte differentiation, and apoptosis. Several transcript variants encoding different protein isoforms have been found for this gene.
UniProt Code:Q9H1Y0
NCBI GenInfo Identifier:17366828
NCBI Gene ID:9474
NCBI Accession:Q9H1Y0.2
UniProt Secondary Accession:Q9H1Y0,O60875, Q5JVR2, Q68DI4, Q9H2B8, Q9HCZ7,
UniProt Related Accession:Q9H1Y0
Molecular Weight:275
NCBI Full Name:Autophagy protein 5
NCBI Synonym Full Names:autophagy related 5
NCBI Official Symbol:ATG5  
NCBI Official Synonym Symbols:ASP; APG5; APG5L; hAPG5; APG5-LIKE  
NCBI Protein Information:autophagy protein 5; apoptosis-specific protein; ATG5 autophagy related 5 homolog
UniProt Protein Name:Autophagy protein 5
UniProt Synonym Protein Names:APG5-like; Apoptosis-specific protein
Protein Family:Autophagy protein
UniProt Gene Name:ATG5  
UniProt Entry Name:ATG5_HUMAN
Anti-ATG5 Antibody (CAB0203)Western blot analysis of extracts of various cell lines, using ATG5 antibody (CAB0203) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
Anti-ATG5 Antibody (CAB0203)Immunohistochemistry of paraffin-embedded Mouse kidney using ATG5 Rabbit pAb (CAB0203) at dilution of 1:50 (40x lens).
Anti-ATG5 Antibody (CAB0203)Immunohistochemistry of paraffin-embedded Rat kidney using ATG5 Rabbit pAb (CAB0203) at dilution of 1:50 (40x lens).
Anti-ATG5 Antibody (CAB0203)Immunohistochemistry of paraffin-embedded Human colon using ATG5 Rabbit pAb (CAB0203) at dilution of 1:50 (40x lens).
Anti-ATG5 Antibody (CAB0203)Immunofluorescence analysis of HeLa cells using ATG5 antibody (CAB0203) at dilution of 1:100. Blue: DAPI for nuclear staining.
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