Antibody Name:	Anti-ATG3 Antibody
Antibody SKU:	CAB5809
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-314 of human ATG3 (NP_071933.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Raji, HeLa, Mouse liver, Mouse heart, Mouse skeletal muscle, Mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-314 of human ATG3 (NP_071933.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MQNV INTV KGKA LEVA EYLT PVLK ESKF KETG VITP EEFV AAGD HLVH HCPT WQWA TGEE LKVK AYLP TGKQ FLVT KNVP CYKR CKQM EYSD ELEA IIEE DDGD GGWV DTYH NTGI TGIT EAVK EITL ENKD NIRL QDCS ALCE EEED EDEG EAAD MEEY EESG LLET DEAT LDTR KIVE ACKA KTDA GGED AILQ TRTY DLYI TYDK YYQT PRLW LFGY DEQR QPLT VEHM YEDI SQDH VKKT VTIE NHPH LPPP PMCS VHPC RHAE VMKK IIET VAEG GGEL GVHM YLLI FLKF VQAV IPTI EYDY TRHF TM
Gene ID:	64422
Uniprot:	Q9NT62
Cellular Location:	Cytoplasm
Calculated MW:	35kDa
Observed MW:	37kDa

Synonyms:

ATG3, APG3, APG3-LIKE, APG3L, PC3-96

Background:

This gene encodes a ubiquitin-like-conjugating enzyme and is a component of ubiquitination-like systems involved in autophagy, the process of degradation, turnover and recycling of cytoplasmic constituents in eukaryotic cells. This protein is known to play a role in regulation of autophagy during cell death. A pseudogene of this gene is located on chromosome 20. Alternative splicing results in multiple transcript variants encoding different isoforms.

UniProt Protein Function:	ATG3: an E2-like enzyme essential for Apg8p lipidation.
UniProt Protein Details:	Protein type:Ubiquitin conjugating system; Enzyme, cellular metabolism; Autophagy; EC 6.3.2.- Chromosomal Location of Human Ortholog: 3q13.2 Cellular Component: cytoplasmic ubiquitin ligase complex; cytosol Molecular Function:APG12 conjugating enzyme activity; APG8 conjugating enzyme activity; enzyme binding; protein binding; small conjugating protein ligase activity Biological Process: autophagic vacuole formation; macroautophagy; mitochondrial fragmentation during apoptosis; mitochondrion degradation; protein modification process; protein targeting to membrane; protein ubiquitination
NCBI Summary:	This gene encodes a ubiquitin-like-conjugating enzyme and is a component of ubiquitination-like systems involved in autophagy, the process of degradation, turnover and recycling of cytoplasmic constituents in eukaryotic cells. This protein is known to play a role in regulation of autophagy during cell death. A pseudogene of this gene is located on chromosome 20. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013]
UniProt Code:	Q9NT62
NCBI GenInfo Identifier:	61212142
NCBI Gene ID:	64422
NCBI Accession:	Q9NT62.1
UniProt Secondary Accession:	Q9NT62,Q6PKC5, Q9H6L9,
UniProt Related Accession:	Q9NT62
Molecular Weight:	35,467 Da
NCBI Full Name:	Ubiquitin-like-conjugating enzyme ATG3
NCBI Synonym Full Names:	autophagy related 3
NCBI Official Symbol:	ATG3
NCBI Official Synonym Symbols:	APG3; APG3L; PC3-96; APG3-LIKE
NCBI Protein Information:	ubiquitin-like-conjugating enzyme ATG3
UniProt Protein Name:	Ubiquitin-like-conjugating enzyme ATG3
UniProt Synonym Protein Names:	Autophagy-related protein 3; APG3-like; hApg3; Protein PC3-96
Protein Family:	Autophagy-related protein
UniProt Gene Name:	ATG3
UniProt Entry Name:	ATG3_HUMAN

	Western blot analysis of extracts of various cell lines, using ATG3 antibody (CAB5809) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded mouse lung using ATG3 antibody (CAB5809) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse spleen using ATG3 antibody (CAB5809) at dilution of 1:200 (40x lens).
	Immunofluorescence analysis of NIH-3T3 cells using ATG3 Rabbit pAb (CAB5809) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.