Autophagy Antibodies
Anti-ATG14 Antibody (CAB7526)
- SKU:
- CAB7526
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Autophagy
Description
| Antibody Name: | Anti-ATG14 Antibody |
| Antibody SKU: | CAB7526 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-310 of human ATG14 (NP_055739.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | MCF7, HeLa, Mouse liver, Mouse brain, Mouse ovary, Rat brain, Rat lung |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-310 of human ATG14 (NP_055739.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MASP SGKG ARAL EAPG CGPR PLAR DLVD SVDD AEGL YVAV ERCP LCNT TRRR LTCA KCVQ SGDF VYFD GRDR ERFI DKKE RLSR LKSK QEEF QKEV LKAM EGKW ITDQ LRWK IMSC KMRI EQLK QTIC KGNE EMEK NSEG LLKT KEKN QKLY SRAQ RHQE KKEK IQRH NRKL GDLV EKKT IDLR SHYE RLAN LRRS HILE LTSV IFPI EEVK TGVR DPAD VSSE SDSA MTSS TVSK LAEA RRTT YLSG RWVC DDHN GDTS ISIT GPWI SLPN NGDY SAYY SWVE EKKT TQGP DMEQ SNPA YTIS AALC YA |
| Gene ID: | 22863 |
| Uniprot: | Q6ZNE5 |
| Cellular Location: | Cytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum membrane, Peripheral membrane protein, Preautophagosomal structure membrane, autophagosome membrane |
| Calculated MW: | 42kDa/55kDa |
| Observed MW: | 60kDa |
| Synonyms: | ATG14, ATG14L, BARKOR, KIAA0831 |
| Background: |
| UniProt Protein Function: | Barkor: Required for both basal and inducible autophagy. Plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine. Promotes BECN1 translocation from the trans-Golgi network to autophagosomes. Enhances PIK3C3 activity in a BECN1-dependent manner. Forms a complex with BECN1, PIK3C3 and PIK3R4, but not with UVRAG, nor with KIAA0226/Rubicon. UVRAG and ATG14/Barkor form mutually exclusive complexes with BECN1 through direct competition. The complex containing ATG14 up-regulates autophagy, while the one containing Rubicon down-regulates autophagy. Interacts with PIK3CB. Belongs to the Barkor family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Autophagy Chromosomal Location of Human Ortholog: 14q22.3 Cellular Component: autophagic vacuole; axoneme; cytoplasm; cytosol; endoplasmic reticulum membrane; nucleus; pre-autophagosomal structure membrane Molecular Function:protein binding Biological Process: autophagic vacuole formation; cellular response to glucose starvation; cellular response to starvation; endosome to lysosome transport; macroautophagy; negative regulation of protein amino acid phosphorylation; positive regulation of phosphoinositide 3-kinase activity; positive regulation of protein amino acid phosphorylation; regulation of protein amino acid phosphorylation |
| UniProt Code: | Q6ZNE5 |
| NCBI GenInfo Identifier: | 67461020 |
| NCBI Gene ID: | 22863 |
| NCBI Accession: | Q6ZNE5.1 |
| UniProt Secondary Accession: | Q6ZNE5,O94920, Q32MK7, Q32MK8, A6NJE4, A8K9U5, B7ZWP5 |
| UniProt Related Accession: | Q6ZNE5 |
| Molecular Weight: | 42,434 Da |
| NCBI Full Name: | Beclin 1-associated autophagy-related key regulator |
| NCBI Synonym Full Names: | autophagy related 14 |
| NCBI Official Symbol: | ATG14 |
| NCBI Official Synonym Symbols: | ATG14L; BARKOR; KIAA0831 |
| NCBI Protein Information: | beclin 1-associated autophagy-related key regulator |
| UniProt Protein Name: | Beclin 1-associated autophagy-related key regulator |
| UniProt Synonym Protein Names: | Autophagy-related protein 14-like protein |
| Protein Family: | Autophagy-related protein |
| UniProt Gene Name: | ATG14 |
| UniProt Entry Name: | BAKOR_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ATG14 antibody (CAB7526) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded rat testis using ATG14 antibody (CAB7526) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of BALB-3T3 cells using ATG14 Rabbit pAb (CAB7526) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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