Name: Anti-ATG13 Antibody (CAB0690)
Brand: Autophagy Antibodies
SKU: CAB0690
Availability: OutOfStock

Antibody Name:	Anti-ATG13 Antibody
Antibody SKU:	CAB0690
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 281-480 of human ATG13 (NP_055556.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, A-549, NCI-H460, SH-SY5Y, Rat brain, Rat heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 281-480 of human ATG13 (NP_055556.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	VHGT QADQ ERLA TCTP SDRT HCAA TPSS SEDT ETVS NSSE GRAS PHDV LETI FVRK VGAF VNKP INQV TLTS LDIP FAMF APKN LELE DTDP MVNP PDSP ETES PLQG SLHS DGSS GGSS GNTH DDFV MIDF KPAF SKDD ILPM DLGT FYRE FQNP PQLS SLSI DIGA QSMA EDLD SLPE KLAV HEKN VREF DAFV ETLQ
Gene ID:	9776
Uniprot:	O75143
Cellular Location:	Cytoplasm, Preautophagosomal structure, cytosol
Calculated MW:	44kDa/52kDa/56kDa/60kDa
Observed MW:	75kDa

Synonyms:	KIAA0652, PARATARG8, ATG13
Background:	The protein encoded by this gene is an autophagy factor and a target of the TOR kinase signaling pathway. The encoded protein is essential for autophagosome formation and mitophagy.

UniProt Protein Function:	ATG13: an autophagy factor required for autophagosome formation. Target of the TOR kinase signaling pathway that regulates autophagy through the control of the phosphorylation status of ATG13 and ULK1, and the regulation of the ATG13-ULK1-RB1CC1 complex. Under starvation conditions, is localized to puncate structures primarily representing the isolation membrane; the isolation membrane sequesters a portion of the cytoplasm resulting in autophagosome formation. Phosphorylated by ULK1 and ULK2. Phosphorylation status depends on nutrient-rich conditions; dephosphorylated during starvation or following treatment with rapamycin. Part of a complex consisting of ATG13, ULK1 and RB1CC1. Interacts with ATG101. Interacts with ULK1 and -2 via C-terminus. Three isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Vesicle; Autophagy Chromosomal Location of Human Ortholog: 11p11.2 Cellular Component: mitochondrion; cytosol Molecular Function:protein binding; protein kinase binding Biological Process: autophagic vacuole formation
NCBI Summary:	The protein encoded by this gene is an autophagy factor and a target of the TOR kinase signaling pathway. The encoded protein is essential for autophagosome formation and mitophagy. [provided by RefSeq, Oct 2016]
UniProt Code:	O75143
NCBI GenInfo Identifier:	20140441
NCBI Gene ID:	9776
NCBI Accession:	O75143.1
UniProt Related Accession:	O75143
Molecular Weight:	Observed: 57 kDaPredicted: 57 kDa
NCBI Full Name:	Autophagy-related protein 13
NCBI Synonym Full Names:	autophagy related 13
NCBI Official Symbol:	ATG13
NCBI Official Synonym Symbols:	KIAA0652; PARATARG8
NCBI Protein Information:	autophagy-related protein 13
UniProt Protein Name:	Autophagy-related protein 13
Protein Family:	Autophagy protein
UniProt Gene Name:	ATG13
UniProt Entry Name:	ATG13_HUMAN

	Western blot analysis of extracts of various cell lines, using ATG13 antibody (CAB0690) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded rat testis using ATG13 antibody (CAB0690) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat brain using ATG13 antibody (CAB0690) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of A549 cells using ATG13 antibody (CAB0690). Blue: DAPI for nuclear staining.