Description
| Antibody Name: | Anti-ATG12 Antibody |
| Antibody SKU: | CAB17045 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-74 of human ATG12 (NP_001264712.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Rat |
| Positive Samples: | rat brain |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-74 of human ATG12 (NP_001264712.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAEE PQSV LQLP TSIA AGGE GLTD VSPE TTTP EPPS SAAV SPGT EEPA GDTK KKIY LCES VLCS FPRP RSWN SL |
| Gene ID: | 9140 |
| Uniprot: | O94817 |
| Cellular Location: | |
| Calculated MW: | 7kDa/15kDa |
| Observed MW: | 55kDa |
| Synonyms: | ATG12, APG12, APG12L, FBR93, HAPG12, Apg12 |
| Background: | Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy (Mizushima et al., 1998 [PubMed 9852036]). |
| UniProt Protein Function: | ATG12: Ubiquitin-like protein required for autophagy. Conjugated to ATG3 and ATG5. Ubiquitous. Belongs to the ATG12 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Autophagy; Ubiquitin-like modifier Chromosomal Location of Human Ortholog: 5q22.3 Cellular Component: cytosol; phagocytic vesicle membrane; pre-autophagosomal structure membrane Molecular Function:APG8 conjugating enzyme activity; protein binding Biological Process: autophagic vacuole formation; C-terminal protein lipidation; macroautophagy; mitochondrion degradation |
| NCBI Summary: | Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy (Mizushima et al., 1998 [PubMed 9852036]).[supplied by OMIM, Mar 2008] |
| UniProt Code: | O94817 |
| NCBI GenInfo Identifier: | 17366241 |
| NCBI Gene ID: | 9140 |
| NCBI Accession: | O94817.1 |
| UniProt Secondary Accession: | O94817,Q6PJV2, |
| UniProt Related Accession: | O94817 |
| Molecular Weight: | 15kDa |
| NCBI Full Name: | Ubiquitin-like protein ATG12 |
| NCBI Synonym Full Names: | autophagy related 12 |
| NCBI Official Symbol: | ATG12 |
| NCBI Official Synonym Symbols: | APG12; FBR93; APG12L; HAPG12 |
| NCBI Protein Information: | ubiquitin-like protein ATG12 |
| UniProt Protein Name: | Ubiquitin-like protein ATG12 |
| UniProt Synonym Protein Names: | Autophagy-related protein 12; APG12-like |
| Protein Family: | Autophagy protein |
| UniProt Gene Name: | ATG12 |
 | Western blot analysis of extracts of rat brain, using ATG12 antibody (CAB17045) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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Additional Information
Product Type: |
Antibody |
Reactivity: |
Rat |
Host Species: |
Rabbit |
Isotype: |
IgG |
Antibody Type: |
Polyclonal Antibody |
Research Area: |
Autophagy |
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