Cell Biology Antibodies 1
Anti-ATF4 Antibody (CAB0201)
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- SKU:
- CAB0201
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-ATF4 Antibody |
| Antibody SKU: | CAB0201 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-351 of human ATF4 (NP_001666.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human |
| Positive Samples: | HeLa |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-351 of human ATF4 (NP_001666.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MTEM SFLS SEVL VGDL MSPF DQSG LGAE ESLG LLDD YLEV AKHF KPHG FSSD KAKA GSSE WLAV DGLV SPSN NSKE DAFS GTDW MLEK MDLK EFDL DALL GIDD LETM PDDL LTTL DDTC DLFA PLVQ ETNK QPPQ TVNP IGHL PESL TKPD QVAP FTFL QPLP LSPG VLSS TPDH SFSL ELGS EVDI TEGD RKPD YTAY VAMI PQCI KEED TPSD NDSG ICMS PESY LGSP QHSP STRG SPNR SLPS PGVL CGSA RPKP YDPP GEKM VAAK VKGE KLDK KLKK MEQN KTAA TRYR QKKR AEQE ALTG ECKE LEKK NEAL KERA DSLA KEIQ YLKD LIEE VRKA RGKK RVP |
| Gene ID: | 468 |
| Uniprot: | P18848 |
| Cellular Location: | Cell membrane, Cytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center |
| Calculated MW: | 38kDa |
| Observed MW: | 50kDa |
| Synonyms: | ATF4, CREB-2, CREB2, TAXREB67, TXREB |
| Background: | This gene encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromosome at q28 in a region containing a large inverted duplication. |
| UniProt Protein Function: | ATF-4: a transcription factor that is a member of the leucine zipper family. Isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). Involved in cisplatin resistance. Other members of the family include AP-1 transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. Two alternatively spliced isoforms have been described. |
| UniProt Protein Details: | Protein type:DNA-binding; Transcription factor Chromosomal Location of Human Ortholog: 22q13.1 Cellular Component: nucleoplasm; neuron projection; cytoplasm; microtubule organizing center; nucleus Molecular Function:protein C-terminus binding; leucine zipper domain binding; protein binding; DNA binding; protein heterodimerization activity; sequence-specific DNA binding; transcription factor activity Biological Process: amino acid metabolic process; transcription from RNA polymerase II promoter; positive regulation of apoptosis; unfolded protein response; positive regulation of transcription, DNA-dependent; negative regulation of potassium ion transport; positive regulation of transcription from RNA polymerase I promoter; gluconeogenesis; cellular response to glucose starvation; unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; regulation of transcription, DNA-dependent; positive regulation of neuron apoptosis; positive regulation of transcription from RNA polymerase II promoter; circadian regulation of gene expression; gamma-aminobutyric acid signaling pathway |
| NCBI Summary: | This gene encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromosome at q28 in a region containing a large inverted duplication. [provided by RefSeq, Sep 2011] |
| UniProt Code: | P18848 |
| NCBI GenInfo Identifier: | 116241262 |
| NCBI Gene ID: | 468 |
| NCBI Accession: | P18848.3 |
| UniProt Secondary Accession: | P18848,Q9UH31, |
| UniProt Related Accession: | P18848 |
| Molecular Weight: | Calculated Molecular Weight: 38kDaObserved Molecular Weight: 38kDa |
| NCBI Full Name: | Cyclic AMP-dependent transcription factor ATF-4 |
| NCBI Synonym Full Names: | activating transcription factor 4 |
| NCBI Official Symbol: | ATF4 |
| NCBI Official Synonym Symbols: | CREB2; TXREB; CREB-2; TAXREB67 |
| NCBI Protein Information: | cyclic AMP-dependent transcription factor ATF-4; DNA-binding protein TAXREB67; tax-responsive enhancer element B67; cAMP response element-binding protein 2; cAMP-dependent transcription factor ATF-4; cAMP-responsive element-binding protein 2; cyclic AMP-responsive element-binding protein 2; tax-responsive enhancer element-binding protein 67 |
| UniProt Protein Name: | Cyclic AMP-dependent transcription factor ATF-4 |
| UniProt Synonym Protein Names: | Activating transcription factor 4; Cyclic AMP-responsive element-binding protein 2; CREB-2; cAMP-responsive element-binding protein 2; DNA-binding protein TAXREB67; Tax-responsive enhancer element-binding protein 67; TaxREB67 |
| Protein Family: | Cyclic AMP-dependent transcription factor |
| UniProt Gene Name: | ATF4 |
| UniProt Entry Name: | ATF4_HUMAN |
 | Western blot analysis of extracts of HeLa cells, using ATF4 antibody (CAB0201) at 1:1000 dilution.HeLa cells were treated by tunicamycin (2 ug/mL) for 8 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 3min. |
 | Immunofluorescence analysis of HeLa cells using ATF4 antibody (CAB0201) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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