Epigenetics & Nuclear Signaling Antibodies 1
Anti-ATF3 Antibody (CAB13469)
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- SKU:
- CAB13469
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-ATF3 Antibody |
| Antibody SKU: | CAB13469 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-181 of human ATF3 (NP_001665.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HepG2, HeLa, RAW264.7, RAW264.7+LPS |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-181 of human ATF3 (NP_001665.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MMLQ HPGQ VSAS EVSA SAIV PCLS PPGS LVFE DFAN LTPF VKEE LRFA IQNK HLCH RMSS ALES VTVS DRPL GVSI TKAE VAPE EDER KKRR RERN KIAA AKCR NKKK EKTE CLQK ESEK LESV NAEL KAQI EELK NEKQ HLIY MLNL HRPT CIVR AQNG RTPE DERN LFIQ QIKE GTLQ S |
| Gene ID: | 467 |
| Uniprot: | P18847 |
| Cellular Location: | Nucleus |
| Calculated MW: | 12kDa/13kDa/14kDa/15kDa/20kDa |
| Observed MW: | 21kDa |
| Synonyms: | ATF3 |
| Background: | This gene encodes a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. This gene is induced by a variety of signals, including many of those encountered by cancer cells, and is involved in the complex process of cellular stress response. Multiple transcript variants encoding different isoforms have been found for this gene. It is possible that alternative splicing of this gene may be physiologically important in the regulation of target genes. |
| UniProt Protein Function: | ATF-3: This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Represses transcription from promoters with ATF sites. It may repress transcription by stabilizing the binding of inhibitory cofactors at the promoter. Isoform 2 activates transcription presumably by sequestering inhibitory cofactors away from the promoters. Belongs to the bZIP family. ATF subfamily. 4 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Transcription factor Chromosomal Location of Human Ortholog: 1q32.3 Cellular Component: nucleolus; nucleoplasm; nucleus Molecular Function:identical protein binding; protein binding; protein heterodimerization activity; protein homodimerization activity; transcription corepressor activity; transcription factor activity Biological Process: negative regulation of transcription from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase II promoter |
| NCBI Summary: | This gene encodes a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. This gene is induced by a variety of signals, including many of those encountered by cancer cells, and is involved in the complex process of cellular stress response. Multiple transcript variants encoding different isoforms have been found for this gene. It is possible that alternative splicing of this gene may be physiologically important in the regulation of target genes. [provided by RefSeq, Apr 2011] |
| UniProt Code: | P18847 |
| NCBI GenInfo Identifier: | 1168543 |
| NCBI Gene ID: | 467 |
| NCBI Accession: | P18847.2 |
| UniProt Secondary Accession: | P18847,Q5VTZ2, Q6ICQ9, Q7Z566, Q8WYM6, |
| UniProt Related Accession: | P18847 |
| Molecular Weight: | 14,314 Da |
| NCBI Full Name: | Cyclic AMP-dependent transcription factor ATF-3 |
| NCBI Synonym Full Names: | activating transcription factor 3 |
| NCBI Official Symbol: | ATF3 |
| NCBI Protein Information: | cyclic AMP-dependent transcription factor ATF-3 |
| UniProt Protein Name: | Cyclic AMP-dependent transcription factor ATF-3 |
| UniProt Synonym Protein Names: | Activating transcription factor 3 |
| Protein Family: | Activating transcription factor |
| UniProt Gene Name: | ATF3 |
| UniProt Entry Name: | ATF3_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ATF3 antibody (CAB13469) at 1:1000 dilution. Raw264.7 cells were treated by LPS (1 ug/mL) at 37'C for 8 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Immunofluorescence analysis of U-2 OS cells using ATF3 antibody (CAB13469) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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