KO Validated Antibodies 1

Anti-ATF2 Antibody (CAB2155)[KO Validated]

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SKU:
CAB2155
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-ATF2 Antibody [KO Validated]
Antibody SKU:CAB2155
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human ATF2 (NP_001871.2).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:Lovo, MCF7, SW480, K-562, 293T
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human ATF2 (NP_001871.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MKFK LHVN SARQ YKDL WNMS DDKP FLCT APGC GQRF TNED HLAV HKHK HEMT LKFG PARN DSVI VADQ TPTP TRFL KNCE EVGL FNEL ASPF ENEF KKAS EDDI KKMP LDLS PLAT PIIR SKIE EPSV VETT HQDS PLPH PEST TSDE KEVP LAQT AQPT SAIV RPAS LQVP NVLL TSSD SSVI IQQA VP
Gene ID:1386
Uniprot:P15336
Cellular Location:Cytoplasm, Mitochondrion outer membrane, Nucleus
Calculated MW:13- 15kDa/23-24kDa/35-54kDa
Observed MW:70kDa
Synonyms:CRE-BP1, CREB-2, CREB2, HB16, TREB7, ATF2
Background:This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions This protein binds to the cAMP-responsive element (CRE), an octameric palindrome. It forms a homodimer or a heterodimer with c-Jun and stimulates CRE-dependent transcription. This protein is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. The encoded protein may also be involved in cell's DNA damage response independent of its role in transcriptional regulation. Several alternatively spliced transcript variants have been found for this gene
UniProt Protein Function:ATF-2: a transcription factor that is a member of the leucine zipper family. Binds to the cAMP-responsive element (CRE). Forms a homodimer or heterodimer with c-Jun and stimulates CRE-dependent transcription. Also possesses histone acetyltransferase (HAT) activity that specifically acetylates histones H2B and H4 in vitro.
UniProt Protein Details:

Protein type:EC 2.3.1.48; Transcription factor; C2H2-type zinc finger protein

Chromosomal Location of Human Ortholog: 2q32

Cellular Component: nucleoplasm; mitochondrial outer membrane; cytoplasm; nucleus

Molecular Function:RNA polymerase II transcription factor activity, enhancer binding; protein binding; histone acetyltransferase activity; protein heterodimerization activity; metal ion binding; cAMP response element binding protein binding; transcription coactivator activity; chromatin binding; transcription factor activity; protein kinase binding

Biological Process: fat cell differentiation; transcription from RNA polymerase II promoter; establishment and/or maintenance of chromatin architecture; MyD88-independent toll-like receptor signaling pathway; stress-activated MAPK cascade; toll-like receptor 3 signaling pathway; positive regulation of transforming growth factor-beta2 production; response to osmotic stress; toll-like receptor 2 signaling pathway; toll-like receptor 10 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; toll-like receptor 5 signaling pathway; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; regulation of transcription factor activity; intra-S DNA damage checkpoint; toll-like receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; histone acetylation; toll-like receptor 9 signaling pathway; response to DNA damage stimulus; toll-like receptor 4 signaling pathway

NCBI Summary:This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions This protein binds to the cAMP-responsive element (CRE), an octameric palindrome. It forms a homodimer or a heterodimer with c-Jun and stimulates CRE-dependent transcription. This protein is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. The encoded protein may also be involved in cell's DNA damage response independent of its role in transcriptional regulation. Several alternatively spliced transcript variants have been found for this gene [provided by RefSeq, Jan 2014]
UniProt Code:P15336
NCBI GenInfo Identifier:215274241
NCBI Gene ID:1386
NCBI Accession:P15336.4
UniProt Secondary Accession:P15336,Q13000, Q3B7B7, Q4ZFU9, Q53RY2, Q8TAR1, A1L3Z2 A4D7U4, A4D7U5, A4D7V1, D3DPE9, G8JLM5,
UniProt Related Accession:P15336
Molecular Weight:505
NCBI Full Name:Cyclic AMP-dependent transcription factor ATF-2
NCBI Synonym Full Names:activating transcription factor 2
NCBI Official Symbol:ATF2  
NCBI Official Synonym Symbols:HB16; CREB2; TREB7; CREB-2; CRE-BP1  
NCBI Protein Information:cyclic AMP-dependent transcription factor ATF-2; histone acetyltransferase ATF2; cAMP-dependent transcription factor ATF-2; cAMP-responsive element-binding protein 2; cAMP response element-binding protein CRE-BP1; cyclic AMP-responsive element-binding protein 2; cAMP responsive element binding protein 2, formerly; activating transcription factor 2 splice variant ATF2-var2
UniProt Protein Name:Cyclic AMP-dependent transcription factor ATF-2
UniProt Synonym Protein Names:Activating transcription factor 2; Cyclic AMP-responsive element-binding protein 2; CREB-2; cAMP-responsive element-binding protein 2; HB16; Histone acetyltransferase ATF2; cAMP response element-binding protein CRE-BP1
Protein Family:Alcohol O-acetyltransferase
UniProt Gene Name:ATF2  
UniProt Entry Name:ATF2_HUMAN
Anti-ATF2 Antibody [KO Validated] (CAB2155)Western blot analysis of extracts of various cell lines, using ATF2 antibody (CAB2155) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Anti-ATF2 Antibody [KO Validated] (CAB2155)Western blot analysis of extracts from normal (control) and ATF2 Rabbit pAb knockout (KO) 293T cells, using ATF2 Rabbit pAb antibody (CAB2155) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-ATF2 Antibody [KO Validated] (CAB2155)Immunohistochemistry of paraffin-embedded rat brain using ATF2 Rabbit pAb (CAB2155) at dilution of 1:100 (40x lens).
Anti-ATF2 Antibody [KO Validated] (CAB2155)Immunohistochemistry of paraffin-embedded mouse spinal cord using ATF2 Rabbit pAb (CAB2155) at dilution of 1:100 (40x lens).
Anti-ATF2 Antibody [KO Validated] (CAB2155)Immunofluorescence analysis of HeLa cells using ATF2 antibody (CAB2155). Blue: DAPI for nuclear staining.
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