Anti-Asymmetric DiMethyl-Histone H3-R2 Antibody (CAB20732)

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SKU:
CAB20732
Product Type:
Antibody
Antibody Type:
Secondary Antibody
Host Species:
Goat
Isotype:
Horseradish peroxidase conjugated IgG

Description

Antibody Name:Anti-Asymmetric DiMethyl-Histone H3-R2 Antibody (CAB20732)
Antibody SKU:CAB20732
Antibody Size:50µL, 100µL
Application:Western blotting
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic asymmetric dimethylated peptide around R2 of human Histone H3 (NP_003520.1).
Application:Western blotting
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa acid extract, NIH/3T3 acid extract, C6 acid extract, H3 protein
Immunogen:A synthetic asymmetric dimethylated peptide around R2 of human Histone H3 (NP_003520.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MART
Cellular Location:Chromosome, Nucleus
Calculated MW:15kDa
Observed MW:17KDa
Synonyms:H3/A, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, H3C10, H3C11, H3C12, HIST1H3A
Background:Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.
Western blot analysis of extracts of various cell lines, using Asymmetric DiMethyl-Histone H3-R2 antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.Western blot analysis of extracts of various cell lines, using Asymmetric DiMethyl-Histone H3-R2 antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
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