Cell Biology Antibodies 6

Anti-AS3MT Antibody (CAB16138)

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SKU:
CAB16138
Product Type:
Antibody
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-AS3MT Antibody
Antibody SKU:CAB16138
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 216-375 of human AS3MT (NP_065733.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Mouse
Positive Samples:Mouse spleen
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 216-375 of human AS3MT (NP_065733.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:LAVL AQKI GFCP PRLV TANL ITIQ NKEL ERVI GDCR FVSA TFRL FKHS KTGP TKRC QVIY NGGI TGHE KELM FDAN FTFK EGEI VEVD EETA AILK NSRF AQDF LIRP IGEK LPTS GGCS ALEL KDII TDPF KLAE ESDS MKSR CVPD AAGG CCGT KKSC
Gene ID:57412
Uniprot:Q9HBK9
Cellular Location:Cytoplasm
Calculated MW:31kDa/41kDa
Observed MW:42kDa
Synonyms:AS3MT, CYT19
Background:AS3MT catalyzes the transfer of a methyl group from S-adenosyl-L-methionine (AdoMet) to trivalent arsenical and may play a role in arsenic metabolism.
UniProt Protein Function:AS3MT: Catalyzes the transfer of a methyl group from AdoMet to trivalent arsenicals producing methylated and dimethylated arsenicals. It methylates arsenite to form methylarsonate, Me- AsO(3)H(2), which is reduced by methylarsonate reductase to methylarsonite, Me-As(OH)2. Methylarsonite is also a substrate and it is converted into the much less toxic compound dimethylarsinate (cacodylate), Me(2)As(O)-OH. Belongs to the methyltransferase superfamily.
UniProt Protein Details:

Protein type:EC 2.1.1.137; Methyltransferase; Mitochondrial

Chromosomal Location of Human Ortholog: 10q24.32

Cellular Component: mitochondrion; cytoplasm; cytosol

Molecular Function:arsenite methyltransferase activity; methylarsonite methyltransferase activity; S-adenosylmethionine-dependent methyltransferase activity

Biological Process: methylation; arsonoacetate metabolic process; toxin metabolic process

NCBI Summary:AS3MT catalyzes the transfer of a methyl group from S-adenosyl-L-methionine (AdoMet) to trivalent arsenical and may play a role in arsenic metabolism (Lin et al., 2002 [PubMed 11790780]).[supplied by OMIM, Mar 2008]
UniProt Code:Q9HBK9
NCBI GenInfo Identifier:109389364
NCBI Gene ID:57412
NCBI Accession:NP_065733.2
UniProt Secondary Accession:Q9HBK9,Q0VDK3, Q0VDK4, Q5PZ02, A6NP79,
UniProt Related Accession:Q9HBK9
Molecular Weight:31,098 Da
NCBI Full Name:arsenite methyltransferase
NCBI Synonym Full Names:arsenite methyltransferase
NCBI Official Symbol:AS3MT  
NCBI Official Synonym Symbols:CYT19  
NCBI Protein Information:arsenite methyltransferase; methyltransferase cyt19; methylarsonite methyltransferase; arsenic (+3 oxidation state) methyltransferase; S-adenosylmethionine:arsenic (III) methyltransferase; S-adenosyl-L-methionine:arsenic(III) methyltransferase
UniProt Protein Name:Arsenite methyltransferase
UniProt Synonym Protein Names:Methylarsonite methyltransferase; S-adenosyl-L-methionine:arsenic(III) methyltransferase
Protein Family:Arsenite methyltransferase
UniProt Gene Name:AS3MT  
UniProt Entry Name:AS3MT_HUMAN
Anti-AS3MT Antibody (CAB16138)Western blot analysis of extracts of mouse spleen, using AS3MT antibody (CAB16138) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
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