Name: Anti-ARPC1A Antibody (CAB14502)
Brand: Cell Biology Antibodies 5
SKU: CAB14502
Availability: OutOfStock

Antibody Name:	Anti-ARPC1A Antibody
Antibody SKU:	CAB14502
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 171-350 of human ARPC1A (NP_006400.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:	Human, Mouse
Positive Samples:	U-251MG

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 171-350 of human ARPC1A (NP_006400.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	AYIK EVDE KPAS TPWG SKMP FGQL MSEF GGSG TGGW VHGV SFSA SGSR LAWV SHDS TVSV ADAS KSVQ VSTL KTEF LPLL SVSF VSEN SVVA AGHD CCPM LFNY DDRG CLTF VSKL DIPK QSIQ RNMS AMER FRNM DKRA TTED RNTA LETL HQNS ITQV SIYE VDKQ DCRK FCTT GIDG
Gene ID:	10552
Uniprot:	Q92747
Cellular Location:	Cytoplasm, cytoskeleton
Calculated MW:	38kDa/41kDa
Observed MW:	42kDa

Synonyms:

ARPC1A, Arc40, HEL-68, HEL-S-307, SOP2Hs, SOP2L

Background:

This gene encodes one of seven subunits of the human Arp2/3 protein complex. This subunit is a member of the SOP2 family of proteins and is most similar to the protein encoded by gene ARPC1B. The similarity between these two proteins suggests that they both may function as p41 subunit of the human Arp2/3 complex that has been implicated in the control of actin polymerization in cells. It is possible that the p41 subunit is involved in assembling and maintaining the structure of the Arp2/3 complex. Multiple versions of the p41 subunit may adapt the functions of the complex to different cell types or developmental stages. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	ARPC1A: Probably functions as component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Belongs to the WD repeat ARPC1 family.
UniProt Protein Details:	Protein type:Cytoskeletal; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 7q22.1 Cellular Component: Arp2/3 protein complex; cytosol; actin cytoskeleton Molecular Function:actin filament binding; actin binding Biological Process: axon guidance; ephrin receptor signaling pathway; innate immune response; actin cytoskeleton organization and biogenesis
NCBI Summary:	This gene encodes one of seven subunits of the human Arp2/3 protein complex. This subunit is a member of the SOP2 family of proteins and is most similar to the protein encoded by gene ARPC1B. The similarity between these two proteins suggests that they both may function as p41 subunit of the human Arp2/3 complex that has been implicated in the control of actin polymerization in cells. It is possible that the p41 subunit is involved in assembling and maintaining the structure of the Arp2/3 complex. Multiple versions of the p41 subunit may adapt the functions of the complex to different cell types or developmental stages. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]
UniProt Code:	Q92747
NCBI GenInfo Identifier:	88984001
NCBI Gene ID:	10552
NCBI Accession:	Q92747.2
UniProt Related Accession:	Q92747
Molecular Weight:	38kDa; 41kDa
NCBI Full Name:	Actin-related protein 2/3 complex subunit 1A
NCBI Synonym Full Names:	actin related protein 2/3 complex subunit 1A
NCBI Official Symbol:	ARPC1A
NCBI Official Synonym Symbols:	Arc40; SOP2L; HEL-68; SOP2Hs; HEL-S-307
NCBI Protein Information:	actin-related protein 2/3 complex subunit 1A
UniProt Protein Name:	Actin-related protein 2/3 complex subunit 1A
UniProt Synonym Protein Names:	SOP2-like protein
Protein Family:	Actin-related protein 2/3 complex
UniProt Gene Name:	ARPC1A
UniProt Entry Name:	ARC1A_HUMAN

Western blot analysis of extracts of U-251MG cells, using ARPC1A antibody (CAB14502) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.

Antibodies

Anti-ARPC1A Antibody (CAB1070)

Anti-ARPC1A Antibody (CAB17079)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)