Cell Biology Antibodies 3
Anti-Aromatase Antibody (CAB12684)
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- SKU:
- CAB12684
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-Aromatase Antibody |
| Antibody SKU: | CAB12684 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human Aromatase (NP_000094.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:1000 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human Aromatase (NP_000094.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MVLE MLNP IHYN ITSI VPEA MPAA TMPV LLLT GLFL LVWN YEGT SSIP GPGY CMGI GPLI SHGR FLWM GIGS ACNY YNRV YGEF MRVW ISGE ETLI ISKS SSMF HIMK HNHY SSRF GSKL GLQC IGMH EKGI IFNN NPEL WKTT RPFF MKAL SGPG LVRM VTVC AESL KTHL DRLE EVTN |
| Gene ID: | 1588 |
| Uniprot: | P11511 |
| Cellular Location: | Membrane, Peripheral membrane protein |
| Calculated MW: | 24kDa/57kDa |
| Observed MW: | 48kDa |
| Synonyms: | CYP19A1, ARO, ARO1, CPV1, CYAR, CYP19, CYPXIX, P-450AROM, aromatase, Aromatase |
| Background: | This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and catalyzes the last steps of estrogen biosynthesis. Mutations in this gene can result in either increased or decreased aromatase activity; the associated phenotypes suggest that estrogen functions both as a sex steroid hormone and in growth or differentiation. Alternative promoter use and alternative splicing results in multiple transcript variants that have different tissue specificities. |
| UniProt Protein Function: | CYP19A1: Catalyzes the formation of aromatic C18 estrogens from C19 androgens. Belongs to the cytochrome P450 family. |
| UniProt Protein Details: | Protein type:EC 1.14.14.14; Oxidoreductase; Lipid Metabolism - androgen and estrogen Chromosomal Location of Human Ortholog: 15q21.1 Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane; membrane Molecular Function:electron carrier activity; heme binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen; oxygen binding; steroid hydroxylase activity Biological Process: estrogen biosynthetic process; steroid biosynthetic process; sterol metabolic process Disease: Aromatase Deficiency; Aromatase Excess Syndrome |
| NCBI Summary: | This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and catalyzes the last steps of estrogen biosynthesis. Mutations in this gene can result in either increased or decreased aromatase activity; the associated phenotypes suggest that estrogen functions both as a sex steroid hormone and in growth or differentiation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014] |
| UniProt Code: | P11511 |
| NCBI GenInfo Identifier: | 117293 |
| NCBI Gene ID: | 1588 |
| NCBI Accession: | P11511.3 |
| UniProt Secondary Accession: | P11511,Q16731, Q3B764, Q58FA0, Q8IYJ7, |
| UniProt Related Accession: | P11511 |
| Molecular Weight: | 24,518 Da |
| NCBI Full Name: | Aromatase |
| NCBI Synonym Full Names: | cytochrome P450 family 19 subfamily A member 1 |
| NCBI Official Symbol: | CYP19A1 |
| NCBI Official Synonym Symbols: | ARO; ARO1; CPV1; CYAR; CYP19; CYPXIX; P-450AROM |
| NCBI Protein Information: | aromatase |
| UniProt Protein Name: | Aromatase |
| UniProt Synonym Protein Names: | CYPXIX; Cytochrome P-450AROM; Cytochrome P450 19A1; Estrogen synthase |
| Protein Family: | Aromatase |
| UniProt Gene Name: | CYP19A1 |
| UniProt Entry Name: | CP19A_HUMAN |
 | Western blot analysis of extracts of various cell lines, using Aromatase antibody (CAB12684) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 20s. |
 | Immunohistochemistry of paraffin-embedded human placenta using Aromatase antibody (CAB12684) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat ovary using Aromatase antibody (CAB12684) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of HeLa cells using Aromatase antibody (CAB12684) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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