Antibody Name:	ARL6 Rabbit Polyclonal Antibody
Antibody SKU:	CAB8269
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-186 of human ARL6 (NP_115522.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:	Human, Mouse
Positive Samples:	293T, Mouse kidney, Mouse brain, Mouse testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-186 of human ARL6 (NP_115522.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MGLL DRLS VLLG LKKK EVHV LCLG LDNS GKTT IINK LKPS NAQS QNIL PTIG FSIE KFKS SSLS FTVF DMSG QGRY RNLW EHYY KEGQ AIIF VIDS SDRL RMVV AKEE LDTL LNHP DIKH RRIP ILFF ANKM DLRD AVTS VKVS QLLC LENI KDKP WHIC ASDA IKGE GLQE GVDW LQDQ IQTV KT
Gene ID:	84100
Uniprot:	Q9H0F7
Cellular Location:	Cell projection, Cytoplasm, Cytoplasmic side, Peripheral membrane protein, cilium axoneme, cilium basal body, cilium membrane, cytoskeleton
Calculated MW:	21kDa
Observed MW:	21kDa

Synonyms:

ARL6, BBS3, RP55

Background:

The protein encoded by this gene belongs to the ARF-like (ADP ribosylation factor-like) sub-family of the ARF family of GTP-binding proteins which are involved in regulation of intracellular traffic. Mutations in this gene are associated with Bardet-Biedl syndrome (BBS). Alternative splicing results in multiple transcript variants of this gene. A vision-specific transcript encoding a different protein has been described (PMID: 20333246).

UniProt Protein Function:	ARL6: Involved in membrane protein trafficking at the base of the ciliary organelle. Mediates recruitment onto plasma membrane of the BBSome complex which would constitute a coat complex required for sorting of specific membrane proteins to the primary cilia. May regulate cilia assembly and disassembly and subsequent ciliary signaling events such as the Wnt signaling cascade. Isoform 2 may be required for proper retinal function and organization. Defects in ARL6 are a cause of Bardet-Biedl syndrome type 3 (BBS3). Bardet-Biedl syndrome (BBS) is a genetically heterogeneous disorder characterized by usually severe pigmentary retinopathy, early onset obesity, polydactyly, hypogenitalism, renal malformation and mental retardation. Secondary features include diabetes mellitus, hypertension and congenital heart disease. Defects in ARL6 are the cause of retinitis pigmentosa type 55 (RP55). RP55 is a retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. Belongs to the small GTPase superfamily. Arf family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:G protein; G protein, monomeric, ARF; G protein, monomeric Chromosomal Location of Human Ortholog: 3q11.2 Cellular Component: axoneme; cilium; cytoplasm; membrane; membrane coat; plasma membrane Molecular Function:phospholipid binding; protein binding Biological Process: cilium biogenesis; determination of left/right symmetry; melanosome transport; protein polymerization; protein targeting to membrane; Wnt receptor signaling pathway Disease: Bardet-biedl Syndrome 1; Bardet-biedl Syndrome 3; Retinitis Pigmentosa; Retinitis Pigmentosa 55
NCBI Summary:	The protein encoded by this gene belongs to the ARF-like (ADP ribosylation factor-like) sub-family of the ARF family of GTP-binding proteins which are involved in regulation of intracellular traffic. Mutations in this gene are associated with Bardet-Biedl syndrome (BBS). A vision-specific transcript, encoding long isoform BBS3L, has been described (PMID: 20333246). [provided by RefSeq, Apr 2016]
UniProt Code:	Q9H0F7
NCBI GenInfo Identifier:	14547903
NCBI Gene ID:	84100
NCBI Accession:	Q9H0F7.1
UniProt Secondary Accession:	Q9H0F7,A8KA93, D3DN31,
UniProt Related Accession:	Q9H0F7
Molecular Weight:	21,960 Da
NCBI Full Name:	ADP-ribosylation factor-like protein 6
NCBI Synonym Full Names:	ADP ribosylation factor like GTPase 6
NCBI Official Symbol:	ARL6
NCBI Official Synonym Symbols:	BBS3; RP55
NCBI Protein Information:	ADP-ribosylation factor-like protein 6
UniProt Protein Name:	ADP-ribosylation factor-like protein 6
UniProt Synonym Protein Names:	Bardet-Biedl syndrome 3 protein
UniProt Gene Name:	ARL6
UniProt Entry Name:	ARL6_HUMAN

	Western blot analysis of extracts of various cell lines, using ARL6 antibody (CAB8269) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human prostate using ARL6 antibody (CAB8269) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human colon using ARL6 antibody (CAB8269) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human liver damage using ARL6 antibody (CAB8269) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse brain using ARL6 antibody (CAB8269) at dilution of 1:100 (40x lens).