Cell Cycle Antibodies 1

Anti-ARL3 Antibody (CAB16348)

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SKU:
CAB16348
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Cycle

Description

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Antibody Name:Anti-ARL3 Antibody
Antibody SKU:CAB16348
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-182 of human ARL3 (NP_004302.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:A-431, LO2, HeLa, Mouse eye, Mouse brain, Mouse heart, Mouse kidney, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-182 of human ARL3 (NP_004302.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MGLL SILR KLKS APDQ EVRI LLLG LDNA GKTT LLKQ LASE DISH ITPT QGFN IKSV QSQG FKLN VWDI GGQR KIRP YWKN YFEN TDIL IYVI DSAD RKRF EETG QELA ELLE EEKL SCVP VLIF ANKQ DLLT AAPA SEIA EGLN LHTI RDRV WQIQ SCSA LTGE GVQD GMNW VCKN VNAK KK
Gene ID:403
Uniprot:P36405
Cellular Location:Cell projection, Cytoplasm, Cytoplasmic side, Golgi apparatus membrane, Nucleus, Peripheral membrane protein, centrosome, cilium, cytoskeleton, microtubule organizing center, spindle
Calculated MW:20kDa
Observed MW:20kDa
Synonyms:ARL3, ARFL3
Background:ADP-ribosylation factor-like 3 is a member of the ADP-ribosylation factor family of GTP-binding proteins. ARL3 binds guanine nucleotides but lacks ADP-ribosylation factor activity.
UniProt Protein Function:ARL3: Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP). Required for normal cytokinesis and cilia signaling. Requires assistance from GTPase- activating proteins (GAPs) like RP2 and PDE6D, in order to cycle between inactive GDP-bound and active GTP-bound forms. Required for targeting proteins such as NPHP3 to the ciliary membrane by releasing myristoylated NPHP3 from UNC119B cargo adapter into the cilium. Does not act as an allosteric activator of the cholera toxin catalytic subunit. Belongs to the small GTPase superfamily. Arf family.
UniProt Protein Details:

Protein type:Cytoskeletal; Cell cycle regulation; G protein, monomeric; G protein, monomeric, ARF

Chromosomal Location of Human Ortholog: 10q23.3

Cellular Component: Golgi membrane; Golgi apparatus; centrosome; cytoplasmic microtubule; spindle microtubule; midbody; nucleus; photoreceptor connecting cilium

Molecular Function:GTPase activity; protein binding; GDP binding; GTP binding; microtubule binding; metal ion binding

Biological Process: photoreceptor cell development; protein transport; smoothened signaling pathway; metabolic process; small GTPase mediated signal transduction; organelle organization and biogenesis; intraflagellar transport; cytokinesis; post-Golgi vesicle-mediated transport; kidney development

NCBI Summary:ADP-ribosylation factor-like 3 is a member of the ADP-ribosylation factor family of GTP-binding proteins. ARL3 binds guanine nucleotides but lacks ADP-ribosylation factor activity. [provided by RefSeq, Jul 2008]
UniProt Code:P36405
NCBI GenInfo Identifier:543851
NCBI Gene ID:403
NCBI Accession:P36405.2
UniProt Secondary Accession:P36405,Q53X83, Q5JSM2, B2R6C7,
UniProt Related Accession:P36405
Molecular Weight:182
NCBI Full Name:ADP-ribosylation factor-like protein 3
NCBI Synonym Full Names:ADP-ribosylation factor-like 3
NCBI Official Symbol:ARL3  
NCBI Official Synonym Symbols:ARFL3  
NCBI Protein Information:ADP-ribosylation factor-like protein 3; ARF-like 3
UniProt Protein Name:ADP-ribosylation factor-like protein 3
Protein Family:ADP-ribosylation factor-like protein
UniProt Gene Name:ARL3  
UniProt Entry Name:ARL3_HUMAN
Anti-ARL3 Antibody (CAB16348)Western blot analysis of extracts of various cell lines, using ARL3 antibody (CAB16348) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-ARL3 Antibody (CAB16348)Immunofluorescence analysis of C6 cells using ARL3 Polyclonal Antibody (CAB16348) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Anti-ARL3 Antibody (CAB16348)Immunofluorescence analysis of L929 cells using ARL3 Polyclonal Antibody (CAB16348) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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