Name: Anti-ARL2BP Antibody (CAB15145)
Brand: Cell Biology Antibodies 5
SKU: CAB15145
Availability: OutOfStock

Antibody Name:	Anti-ARL2BP Antibody
Antibody SKU:	CAB15145
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ARL2BP (NP_036238.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human
Positive Samples:	U-251MG, MCF7

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ARL2BP (NP_036238.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MDAL EGES FALS FSSA SDAE FDAV VGYL EDII MDDE FQLL QRNF MDKY YLEF EDTE ENKL IYTP IFNE YISL VEKY IEEQ LLQR IPEF NMAA FTTT LQHH
Gene ID:	23568
Uniprot:	Q9Y2Y0
Cellular Location:	Cytoplasm, Mitochondrion intermembrane space, Nucleus, centrosome, cilium basal body, cytoskeleton, microtubule organizing center, spindle
Calculated MW:	17kDa/18kDa
Observed MW:	19kDa

Synonyms:

ARL2BP, BART, BART1, RP66

Background:

ADP-ribosylation factor (ARF)-like proteins (ARLs) comprise a functionally distinct group of the ARF family of RAS-related GTPases. The protein encoded by this gene binds to ARL2.GTP with high affinity but does not interact with ARL2.GDP, activated ARF, or RHO proteins. The lack of detectable membrane association of this protein or ARL2 upon activation of ARL2 is suggestive of actions distinct from those of the ARFs. This protein is considered to be the first ARL2-specific effector identified, due to its interaction with ARL2.GTP but lack of ARL2 GTPase-activating protein activity.

UniProt Protein Function:	ARL2BP: Together with ARL2, plays a role in the nuclear translocation, retention and transcriptional activity of STAT3. May play a role as an effector of ARL2. Belongs to the ARL2BP family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:G protein regulator, misc.; Mitochondrial Chromosomal Location of Human Ortholog: 16q13 Cellular Component: centrosome; cytosol; midbody; mitochondrial intermembrane space; mitochondrial matrix Molecular Function:GTPase regulator activity; protein binding; transcription coactivator activity Biological Process: maintenance of protein localization in nucleus; positive regulation of tyrosine phosphorylation of STAT protein; regulation of insulin secretion; signal transduction Disease: Retinitis Pigmentosa With Or Without Situs Inversus
NCBI Summary:	ADP-ribosylation factor (ARF)-like proteins (ARLs) comprise a functionally distinct group of the ARF family of RAS-related GTPases. The protein encoded by this gene binds to ARL2.GTP with high affinity but does not interact with ARL2.GDP, activated ARF, or RHO proteins. The lack of detectable membrane association of this protein or ARL2 upon activation of ARL2 is suggestive of actions distinct from those of the ARFs. This protein is considered to be the first ARL2-specific effector identified, due to its interaction with ARL2.GTP but lack of ARL2 GTPase-activating protein activity. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9Y2Y0
NCBI GenInfo Identifier:	6912268
NCBI Gene ID:	23568
NCBI Accession:	NP_036238.1
UniProt Secondary Accession:	Q9Y2Y0,Q504R0, B3KQJ5,
UniProt Related Accession:	Q9Y2Y0
Molecular Weight:	46.2kD
NCBI Full Name:	ADP-ribosylation factor-like protein 2-binding protein
NCBI Synonym Full Names:	ADP ribosylation factor like GTPase 2 binding protein
NCBI Official Symbol:	ARL2BP
NCBI Official Synonym Symbols:	BART; RP66; BART1
NCBI Protein Information:	ADP-ribosylation factor-like protein 2-binding protein
UniProt Protein Name:	ADP-ribosylation factor-like protein 2-binding protein
UniProt Synonym Protein Names:	Binder of ARF2 protein 1
Protein Family:	ADP-ribosylation factor-like protein
UniProt Gene Name:	ARL2BP

Western blot analysis of extracts of various cell lines, using ARL2BP antibody (CAB15145) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Antibodies

ARL2BP Antibody (PACO00456)

ARL2BP Antibody (PACO07842)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)