Antibody Name:	Anti-ARL1 Antibody
Antibody SKU:	CAB15254
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 62-181 of human ARL1 (NP_001168.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:200 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, A-549, Mouse thymus, Mouse brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 62-181 of human ARL1 (NP_001168.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	KFQV WDLG GQTS IRPY WRCY YSNT DAVI YVVD SCDR DRIG ISKS ELVA MLEE EELR KAIL VVFA NKQD MEQA MTSS EMAN SLGL PALK DRKW QIFK TSAT KGTG LDEA MEWL VETL KSRQ
Gene ID:	400
Uniprot:	P40616
Cellular Location:	Cytoplasmic side, Golgi apparatus membrane, Lipid-anchor, Membrane, Peripheral membrane protein
Calculated MW:	18kDa/20kDa
Observed MW:	20kDa

Synonyms:

ARL1, ARFL1

Background:

The protein encoded by this gene belongs to the ARL (ADP-ribosylation factor-like) family of proteins, which are structurally related to ADP-ribosylation factors (ARFs). ARFs, described as activators of cholera toxin (CT) ADP-ribosyltransferase activity, regulate intracellular vesicular membrane trafficking, and stimulate a phospholipase D (PLD) isoform. Although, ARL proteins were initially thought not to activate CT or PLD, later work showed that they are weak stimulators of PLD and CT in a phospholipid dependent manner. Alternative splicing results in multiple transcript variants encoding different isoforms.

UniProt Protein Function:	ARL1: GTP-binding protein that has very low efficiency as allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Can activate phospholipase D with very low efficiency. Important for normal function of the Golgi apparatus. Belongs to the small GTPase superfamily. Arf family.
UniProt Protein Details:	Protein type:G protein; G protein, monomeric; G protein, monomeric, ARF Chromosomal Location of Human Ortholog: 12q23.2 Cellular Component: Golgi membrane; Golgi apparatus; cytoplasm; trans-Golgi network Molecular Function:GTPase activity; protein domain specific binding; GTP binding; metal ion binding; enzyme activator activity Biological Process: phospholipase D activation; toxin metabolic process; protein localization in Golgi apparatus; small GTPase mediated signal transduction; retrograde transport, endosome to Golgi; Golgi organization and biogenesis; Golgi vesicle transport
NCBI Summary:	The protein encoded by this gene belongs to the ARL (ADP-ribosylation factor-like) family of proteins, which are structurally related to ADP-ribosylation factors (ARFs). ARFs, described as activators of cholera toxin (CT) ADP-ribosyltransferase activity, regulate intracellular vesicular membrane trafficking, and stimulate a phospholipase D (PLD) isoform. Although, ARL proteins were initially thought not to activate CT or PLD, later work showed that they are weak stimulators of PLD and CT in a phospholipid dependent manner. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2014]
UniProt Code:	P40616
NCBI GenInfo Identifier:	728888
NCBI Gene ID:	400
NCBI Accession:	P40616.1
UniProt Related Accession:	P40616
Molecular Weight:	20kDa
NCBI Full Name:	ADP-ribosylation factor-like protein 1
NCBI Synonym Full Names:	ADP ribosylation factor like GTPase 1
NCBI Official Symbol:	ARL1
NCBI Official Synonym Symbols:	ARFL1
NCBI Protein Information:	ADP-ribosylation factor-like protein 1
UniProt Protein Name:	ADP-ribosylation factor-like protein 1
Protein Family:	ARL14 effector protein
UniProt Gene Name:	ARL1
UniProt Entry Name:	ARL1_HUMAN

	Western blot analysis of extracts of various cell lines, using ARL1 antibody (CAB15254) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3min.
	Immunofluorescence analysis of C6 cells using ARL1 Polyclonal Antibody (CAB15254) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of L929 cells using ARL1 Polyclonal Antibody (CAB15254) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.