Name: Anti-ARID3A Antibody (CAB7668)
Brand: ChIP Antibodies
SKU: CAB7668
Availability: OutOfStock

Antibody Name:	Anti-ARID3A Antibody
Antibody SKU:	CAB7668
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 324-593 of human ARID3A (NP_005215.1).

Application:	WB IHC IP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IP 1:20 - 1:50
Reactivity:	Human, Mouse, Rat
Positive Samples:	Jurkat, MCF7, Mouse testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 324-593 of human ARID3A (NP_005215.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	PYEC EKRG LSNP NELQ AAID SNRR EGRR QSFG GSLF AYSP GGAH GMLS SPKL PVSS LGLA ASTN GSSI TPAP KIKK EEDS AIPI TVPG RLPV SLAG HPVV AAQA AAVQ AAAA QAAV AAQA AALE QLRE KLES AEPP EKKM ALVA DEQQ RLMQ RALQ QNFL AMAA QLPM SIRI NSQA SESR QDSA VNLT GTNG SNSI SMSV EING IMYT GVLF AQPP APTP TSAP NKGG GGGG GSSS NAGG RGGN TGTS GGQA GPAG LSTP STST SNNS LP
Gene ID:	1820
Uniprot:	Q99856
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	62kDa
Observed MW:	95kDa

Synonyms:	ARID3A, BRIGHT, DRIL1, DRIL3, E2FBP1
Background:	This gene encodes a member of the ARID (AT-rich interaction domain) family of DNA binding proteins. It was found by homology to the Drosophila dead ringer gene, which is important for normal embryogenesis. Other ARID family members have roles in embryonic patterning, cell lineage gene regulation, cell cycle control, transcriptional regulation, and possibly in chromatin structure modification.

UniProt Protein Function:	Bright: a member of the ARID (AT-rich interaction domain) family of transcription factors. It was found by its homology to the Drosophila dead ringer protein, which is important for normal embryogenesis. Binds a VH promoter proximal site necessary for induced mu-heavy-chain transcription. Binds the minor groove of a restricted ATC sequence that is sufficient for nuclear matrix association. This sequence motif is present in matrix-associating regions (MARS) proximal to the promoter and flanking E mu. Activates E mu-driven transcription by binding these sites. Contributes to localized control of accessibility and therefore nonrandom gene use during V(D)J recombination.
UniProt Protein Details:	Protein type:Transcription factor Chromosomal Location of Human Ortholog: 19p13.3 Cellular Component: nucleoplasm; Golgi apparatus; cytoplasm; nucleolus; lipid raft Molecular Function:protein binding; protein homodimerization activity; chromatin binding Biological Process: transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter
NCBI Summary:	This gene encodes a member of the ARID (AT-rich interaction domain) family of DNA binding proteins. It was found by homology to the Drosophila dead ringer gene, which is important for normal embryogenesis. Other ARID family members have roles in embryonic patterning, cell lineage gene regulation, cell cycle control, transcriptional regulation, and possibly in chromatin structure modification. [provided by RefSeq, Jul 2008]
UniProt Code:	Q99856
NCBI GenInfo Identifier:	12230034
NCBI Gene ID:	1820
NCBI Accession:	Q99856.2
UniProt Related Accession:	Q99856
Molecular Weight:
NCBI Full Name:	AT-rich interactive domain-containing protein 3A
NCBI Synonym Full Names:	AT-rich interaction domain 3A
NCBI Official Symbol:	ARID3A
NCBI Official Synonym Symbols:	DRIL1; DRIL3; BRIGHT; E2FBP1
NCBI Protein Information:	AT-rich interactive domain-containing protein 3A
UniProt Protein Name:	AT-rich interactive domain-containing protein 3A
UniProt Synonym Protein Names:	B-cell regulator of IgH transcription; Bright; Dead ringer-like protein 1; E2F-binding protein 1
UniProt Gene Name:	ARID3A
UniProt Entry Name:	ARI3A_HUMAN

	Western blot analysis of extracts of various cell lines, using ARID3A antibody (CAB7668) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat lung using ARID3A Rabbit pAb (CAB7668) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human lung cancer using ARID3A Rabbit pAb (CAB7668) at dilution of 1:100 (40x lens).
	Immunoprecipitation analysis of 150ug extracts of MCF7 cells using 3ug ARID3A antibody (CAB7668). Western blot was performed from the immunoprecipitate using ARID3A antibody (CAB7668) at a dilition of 1:500.