KO Validated Antibodies 1

Anti-ARID2 Antibody (CAB8601)[KO Validated]

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SKU:
CAB8601
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-ARID2 Antibody [KO Validated]
Antibody SKU:CAB8601
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1586-1835 of human ARID2 (NP_689854.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:LO2
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1586-1835 of human ARID2 (NP_689854.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:QNVV PQNT PMPP SPAV QVQG QPNS SQPS PFSG SSQP GDPM RKPG QNFM CLWQ SCKK WFQT PSQV FYHA ATEH GGKD VYPG QCLW EGCE PFQR QRFS FITH LQDK HCSK DALL AGLK QDEP GQAG SQKS STKQ PTVG GTSS TPRA QKAI VNHP SAAL MALR RGSR NLVF RDFT DEKE GPIT KHIR LTAA LILK NIGK YSEC GRRL LKRH ENNL SVLA ISNM EASS TLAK CLYE LNFT VQSK EQEK DSEM LQ
Gene ID:196528
Uniprot:Q68CP9
Cellular Location:Nucleus
Calculated MW:191kDa/197kDa
Observed MW:250kDa
Synonyms:ARID2, BAF200, p200
Background:
UniProt Protein Function:ARID2: Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Required for the stability of the SWI/SNF chromatin remodeling complex SWI/SNF-B (PBAF). May be involved in targeting the complex to different genes. May be involved in regulating transcriptional activation of cardiac genes. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:C2H2-type zinc finger protein

Chromosomal Location of Human Ortholog: 12q12

Cellular Component: nucleoplasm; plasma membrane

Molecular Function:protein binding; DNA binding; metal ion binding

Biological Process: regulation of transcription, DNA-dependent; nucleosome disassembly; transcription, DNA-dependent; chromatin modification

NCBI Summary:ARID2 is a subunit of the PBAF chromatin-remodeling complex (see BAF180; MIM 606083), which facilitates ligand-dependent transcriptional activation by nuclear receptors (Yan et al., 2005 [PubMed 15985610]).[supplied by OMIM, Mar 2008]
UniProt Code:Q68CP9
NCBI GenInfo Identifier:73921721
NCBI Gene ID:196528
NCBI Accession:Q68CP9.2
UniProt Secondary Accession:Q68CP9,Q15KG9, Q5EB51, Q645I3, Q6ZRY5, Q7Z3I5, Q86T28 Q96SJ6, Q9HCL5,
UniProt Related Accession:Q68CP9
Molecular Weight:191,422 Da
NCBI Full Name:AT-rich interactive domain-containing protein 2
NCBI Synonym Full Names:AT rich interactive domain 2 (ARID, RFX-like)
NCBI Official Symbol:ARID2  
NCBI Official Synonym Symbols:p200; BAF200  
NCBI Protein Information:AT-rich interactive domain-containing protein 2; zipzap; zipzap/p200; BRG1-associated factor 200; ARID domain-containing protein 2; zinc finger protein with activation potential
UniProt Protein Name:AT-rich interactive domain-containing protein 2
UniProt Synonym Protein Names:BRG1-associated factor 200; BAF200; Zinc finger protein with activation potential; Zipzap/p200
Protein Family:AT-rich interactive domain-containing protein
UniProt Gene Name:ARID2  
UniProt Entry Name:ARID2_HUMAN
Anti-ARID2 Polyclonal Antibody [KO Validated] (CAB8601)Western blot analysis of extracts of LO2 cells, using ARID2 antibody (CAB8601) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-ARID2 Polyclonal Antibody [KO Validated] (CAB8601)Western blot analysis of extracts from normal (control) and ARID2 knockout (KO) HeLa cells, using ARID2 antibody (CAB8601) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
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