Antibody Name:	Anti-ARID1B Antibody
Antibody SKU:	CAB15488
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 400-650 of human ARID1B (NP_059989.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:200 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 400-650 of human ARID1B (NP_059989.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	GGFQ RFAG QNQH PSGA TPTL NQLL TSPS PMMR SYGG SYPE YSSP SAPP PPPS QPQS QAAA AGAA AGGQ QAAA GMGL GKDM GAQY AAAS PAWA AAQQ RSHP AMSP GTPG PTMG RSQG SPMD PMVM KRPQ LYGM GSNP HSQP QQSS PYPG GSYG PPGP QRYP IGIQ GRTP GAMA GMQY PQQQ MPPQ YGQQ GVSG YCQQ GQQP YYSQ QPQP PHLP PQAQ YLPS QSQQ RYQP QQDM SQEG YGTR SQPP LAPG KPN
Gene ID:	57492
Uniprot:	Q8NFD5
Cellular Location:	Nucleus
Calculated MW:	162kDa/236kDa/237kDa/241kDa
Observed MW:	Refer to figures

Synonyms:

ARID1B, 6A3-5, BAF250B, BRIGHT, CSS1, DAN15, ELD/OSA1, MRD12, OSA2, P250R

Background:

This locus encodes an AT-rich DNA interacting domain-containing protein. The encoded protein is a component of the SWI/SNF chromatin remodeling complex and may play a role in cell-cycle activation. The protein encoded by this locus is similar to AT-rich interactive domain-containing protein 1A. These two proteins function as alternative, mutually exclusive ARID-subunits of the SWI/SNF complex. The associated complexes play opposing roles. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	ARID1B: Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. Binds DNA non-specifically. Defects in ARID1B are the cause of mental retardation autosomal dominant type 12 (MRD12). A disorder characterized by significantly below average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. MRD12 patients present with moderate to severe psychomotor retardation, and most show evidence of muscular hypotonia. In many patients, expressive speech is more severely affected than receptive function. Additional common findings include short stature, abnormal head shape and low-set, posteriorly rotated, and abnormally shaped ears, downslanting palpebral fissures, a bulbous nasal tip, a thin upper lip, minor teeth anomalies, and brachydactyly or single palmar creases. Autistic features are uncommon. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Nuclear receptor co-regulator; DNA-binding Chromosomal Location of Human Ortholog: 6q25.1 Cellular Component: nucleoplasm; SWI/SNF complex; cytoplasm Molecular Function:protein binding; DNA binding; transcription coactivator activity Biological Process: nervous system development; transcription, DNA-dependent; chromatin-mediated maintenance of transcription Disease: Mental Retardation, Autosomal Dominant 12
NCBI Summary:	This locus encodes an AT-rich DNA interacting domain-containing protein. The encoded protein is a component of the SWI/SNF chromatin remodeling complex and may play a role in cell-cycle activation. The protein encoded by this locus is similar to AT-rich interactive domain-containing protein 1A. These two proteins function as alternative, mutually exclusive ARID-subunits of the SWI/SNF complex. The associated complexes play opposing roles. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Feb 2012]
UniProt Code:	Q8NFD5
NCBI GenInfo Identifier:	73921720
NCBI Gene ID:	57492
NCBI Accession:	Q8NFD5.2
UniProt Secondary Accession:	Q8NFD5,Q5JRD1, Q5VYC4, Q8IZY8, Q8TEV0, Q8TF02, Q99491 Q9ULI5,
UniProt Related Accession:	Q8NFD5
Molecular Weight:	162,470 Da
NCBI Full Name:	AT-rich interactive domain-containing protein 1B
NCBI Synonym Full Names:	AT rich interactive domain 1B (SWI1-like)
NCBI Official Symbol:	ARID1B
NCBI Official Synonym Symbols:	OSA2; 6A3-5; DAN15; MRD12; P250R; BRIGHT; BAF250B; ELD/OSA1
NCBI Protein Information:	AT-rich interactive domain-containing protein 1B; ELD (eyelid)/OSA protein; BRG1-associated factor 250b; BRG1-binding protein ELD/OSA1; ARID domain-containing protein 1B
UniProt Protein Name:	AT-rich interactive domain-containing protein 1B
UniProt Synonym Protein Names:	BRG1-associated factor 250b; BAF250B; BRG1-binding protein hELD/OSA1; Osa homolog 2; hOsa2; p250R
Protein Family:	AT-rich interactive domain-containing protein
UniProt Gene Name:	ARID1B
UniProt Entry Name:	ARI1B_HUMAN

	Western blot analysis of extracts of various cell lines, using ARID1B antibody (CAB15488) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded Mouse brain using ARID1B Rabbit pAb (CAB15488) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded Rat heart using ARID1B Rabbit pAb (CAB15488) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded Human breast using ARID1B Rabbit pAb (CAB15488) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of C6 cells using ARID1B antibody (CAB15488) at dilution of 1:100. Blue: DAPI for nuclear staining.