Name: Anti-ARHGEF7 Antibody (CAB1108)
Brand: Neuroscience
SKU: CAB1108
Availability: OutOfStock

Antibody Name:	Anti-ARHGEF7 Antibody
Antibody SKU:	CAB1108
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Monkey
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human ARHGEF7 (NP_001106984.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Monkey
Positive Samples:	SW620, Jurkat, COS-1, COS-7, MCF7, K-562, Mouse lung, Mouse heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human ARHGEF7 (NP_001106984.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MNSA EQTV TWLI TLGV LESP KKTI SDPE GFLQ ASLK DGVV LCRL LERL LPGT IEKT FDAN DLYQ GQNF NKVL SSLV TLNK VTAD IGLG SDSV CARP SSHR IKSF DSLG SQSL HTRT SKLF QGQY RSLD MTDN SNNQ LVVR AKFN FQQT NEDE LSFS KGDV IHVT RVEE GGWW EGTL NGRT GWFP SNYV REVK ASEK PVSP KSGT LKSP PKGF DTTA INKS YYNV VLQN ILET ENEY SKEL QTVL STYL RPLQ TSEK LSSA
Gene ID:	8874
Uniprot:	Q14155
Cellular Location:	Cell junction, Cell projection, Cytoplasm, cell cortex, focal adhesion, lamellipodium, ruffle
Calculated MW:	70kDa/73kDa/82kDa/84kDa/87kDa/90kDa
Observed MW:	86kDa

Synonyms:	ARHGEF7, BETA-PIX, COOL-1, COOL1, Nbla10314, P50, P50BP, P85, P85COOL1, P85SPR, PAK3, PIXB
Background:	This gene encodes a protein that belongs to a family of cytoplasmic proteins that activate the Ras-like family of Rho proteins by exchanging bound GDP for GTP. It forms a complex with the small GTP binding protein Rac1 and recruits Rac1 to membrane ruffles and to focal adhesions. Multiple alternatively spliced transcript variants encoding different isoforms have been observed for this gene.

UniProt Protein Function:	ARHGEF7: a RAC1 guanine nucleotide exchange factor (GEF). Can induce membrane ruffling. Interacts with PAK kinases through its SH3 domain. Acts both as an activator and a downstream target for Cdc42. Its EGF-dependent phosphorylation activates its nucleotide exchange factor for Cdc42 and regulates its interaction with the E3 ligase Cbl, thus modulating Cbl-catalysed EGFR degradation. Its phosphorylation status is necessary to maintain the balance between normal signaling by EGFR and Src versus aberrant growth and transformation. Four alternatively spliced isoforms have been described.
UniProt Protein Details:	Protein type:GEFs; GEFs, Rac/Rho; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 13q34 Cellular Component: cell cortex; cell soma; cytosol; focal adhesion; lamellipodium; neuron projection; plasma membrane; protein complex; ruffle Molecular Function:guanyl-nucleotide exchange factor activity; protein binding; protein kinase binding; Rho guanyl-nucleotide exchange factor activity Biological Process: ephrin receptor signaling pathway; focal adhesion formation; Golgi organization and biogenesis; hemopoietic progenitor cell differentiation; lamellipodium biogenesis; negative regulation of epidermal growth factor receptor signaling pathway; nervous system development; positive regulation of apoptosis; positive regulation of growth hormone secretion; positive regulation of GTPase activity; positive regulation of protein binding; regulation of Rho protein signal transduction; regulation of small GTPase mediated signal transduction; signal transduction
NCBI Summary:	This gene encodes a protein that belongs to a family of cytoplasmic proteins that activate the Ras-like family of Rho proteins by exchanging bound GDP for GTP. It forms a complex with the small GTP binding protein Rac1 and recruits Rac1 to membrane ruffles and to focal adhesions. Multiple alternatively spliced transcript variants encoding different isoforms have been observed for this gene. [provided by RefSeq, Mar 2016]
UniProt Code:	Q14155
NCBI GenInfo Identifier:	50403776
NCBI Gene ID:	8874
NCBI Accession:	Q14155.2
UniProt Secondary Accession:	Q14155,Q3LIA4, Q5W9H0, Q6P9G3, Q6PII2, Q86W63, Q8N3M1 B1ALK6, B1ALK8,
UniProt Related Accession:	Q14155
Molecular Weight:	70,450 Da
NCBI Full Name:	Rho guanine nucleotide exchange factor 7
NCBI Synonym Full Names:	Rho guanine nucleotide exchange factor 7
NCBI Official Symbol:	ARHGEF7
NCBI Official Synonym Symbols:	P50; P85; PAK3; PIXB; COOL1; P50BP; COOL-1; P85SPR; BETA-PIX; P85COOL1; Nbla10314
NCBI Protein Information:	rho guanine nucleotide exchange factor 7
UniProt Protein Name:	Rho guanine nucleotide exchange factor 7
UniProt Synonym Protein Names:	Beta-Pix; COOL-1; PAK-interacting exchange factor beta; p85
Protein Family:	Rho guanine nucleotide exchange factor
UniProt Gene Name:	ARHGEF7
UniProt Entry Name:	ARHG7_HUMAN

Western blot analysis of extracts of various cell lines, using ARHGEF7 antibody (CAB1108) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Antibodies

ARHGEF7 Antibody (PACO05638)

ARHGEF7 Antibody (PACO07835)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)