KO Validated Antibodies 1

Anti-ARHGEF1 Antibody (CAB4274)[KO Validated]

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SKU:
CAB4274
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-ARHGEF1 Antibody [KO Validated]
Antibody SKU:CAB4274
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 783-912 of human ARHGEF1 (NP_004697.2).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:200
Reactivity:Human, Rat
Positive Samples:HeLa, Jurkat, LO2
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 783-912 of human ARHGEF1 (NP_004697.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:PLLS SSEN GNGG RETS PADA RTER ILSD LLPF CRPG PEGQ LAAT ALRK VLSL KQLL FPAE EDNG AGPP RDGD GVPG GGPL SPAR TQEI QENL LSLE ETMK QLEE LEEE FCRL RPLL SQLG GNSV PQPG CT
Gene ID:9138
Uniprot:Q92888
Cellular Location:Cytoplasm, Membrane
Calculated MW:98kDa/102kDa/104kDa/105kDa
Observed MW:102kDa
Synonyms:ARHGEF1, GEF1, LBCL2, LSC, P115-RHOGEF, SUB1.5
Background:Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate Rho-dependent signals. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined.
UniProt Protein Function:Seems to play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13) subunits. Acts as GTPase-activating protein (GAP) for GNA12 and GNA13, and as guanine nucleotide exchange factor (GEF) for RhoA GTPase. Activated G alpha 13/GNA13 stimulates the RhoGEF activity through interaction with the RGS-like domain. This GEF activity is inhibited by binding to activated GNA12. Mediates angiotensin-2-induced RhoA activation.
NCBI Summary:Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate Rho-dependent signals. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined. [provided by RefSeq, Jul 2008]
UniProt Code:Q92888
NCBI GenInfo Identifier:34395524
NCBI Gene ID:9138
NCBI Accession:Q92888.2
UniProt Secondary Accession:Q92888,O00513, Q6NX52, Q8N4J4, Q96BF4, Q96F17, Q9BSB1
UniProt Related Accession:Q92888
Molecular Weight:105,854 Da
NCBI Full Name:Rho guanine nucleotide exchange factor 1
NCBI Synonym Full Names:Rho guanine nucleotide exchange factor 1
NCBI Official Symbol:ARHGEF1  
NCBI Official Synonym Symbols:LSC; GEF1; LBCL2; SUB1.5; P115-RHOGEF  
NCBI Protein Information:rho guanine nucleotide exchange factor 1
UniProt Protein Name:Rho guanine nucleotide exchange factor 1
UniProt Synonym Protein Names:115 kDa guanine nucleotide exchange factor; p115-RhoGEF; p115RhoGEF; Sub1.5
Protein Family:Rho guanine nucleotide exchange factor
UniProt Gene Name:ARHGEF1  
UniProt Entry Name:ARHG1_HUMAN
Anti-ARHGEF1 Antibody [KO Validated] (CAB4274)Western blot analysis of extracts of various cell lines, using ARHGEF1 antibody (CAB4274) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-ARHGEF1 Antibody [KO Validated] (CAB4274)Western blot analysis of extracts from normal (control) and ARHGEF1 knockout (KO) 293T cells, using ARHGEF1 antibody (CAB4274) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Anti-ARHGEF1 Antibody [KO Validated] (CAB4274)Immunohistochemistry of paraffin-embedded human tonsil using ARHGEF1 antibody (CAB4274) at dilution of 1:100 (40x lens).
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