KO Validated Antibodies 1

Anti-ARHGDIA Antibody (CAB1214)[KO Validated]

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SKU:
CAB1214
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-ARHGDIA Antibody [KO Validated]
Antibody SKU:CAB1214
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-204 of human ARHGDIA (NP_001172006.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:293T, HeLa, COS7, PC-12, Mouse lung, Mouse spleen, Mouse kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-204 of human ARHGDIA (NP_001172006.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MAEQ EPTA EQLA QIAA ENEE DEHS VNYK PPAQ KSIQ EIQE LDKD DESL RKYK EALL GRVA VSAD PNVP NVVV TGLT LVCS SAPG PLEL DLTG DLES FKKQ SFVL KEGV EYRI KISF RVNR EIVS GMKY IQHT YRKG VKID KTDY MVGS YGPR AEEY EFLT PVEE APKG MLAR GSYS IKSR FTDD DKTD HLSW EWNL TIKK DWKD
Gene ID:396
Uniprot:P52565
Cellular Location:Cytoplasm
Calculated MW:18kDa/23kDa
Observed MW:25kDa
Synonyms:ARHGDIA, GDIA1, HEL-S-47e, NPHS8, RHOGDI, RHOGDI-1
Background:This gene encodes a protein that plays a key role in the regulation of signaling through Rho GTPases. The encoded protein inhibits the disassociation of Rho family members from GDP (guanine diphosphate), thereby maintaining these factors in an inactive state. Activity of this protein is important in a variety of cellular processes, and expression of this gene may be altered in tumors. Mutations in this gene have been found in individuals with nephrotic syndrome, type 8. Alternate splicing results in multiple transcript variants.
UniProt Protein Function:RhoGDI alpha: Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. Monomer. Forms a heterodimer with RAC1. Interacts with FER. Belongs to the Rho GDI family.
UniProt Protein Details:

Protein type:Apoptosis; Cell development/differentiation; G protein regulator, misc.; Cell adhesion; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 17q25.3

Cellular Component: cytoskeleton; cytosol

Molecular Function:GTPase activator activity; protein binding; Rho GDP-dissociation inhibitor activity

Biological Process: cell motility; negative regulation of apoptosis; negative regulation of axonogenesis; negative regulation of cell adhesion; nerve growth factor receptor signaling pathway; positive regulation of axonogenesis; positive regulation of GTPase activity; regulation of axonogenesis; regulation of catalytic activity; regulation of small GTPase mediated signal transduction; Rho protein signal transduction; small GTPase mediated signal transduction

Disease: Nephrotic Syndrome, Type 8

NCBI Summary:This gene encodes a protein that plays a key role in the regulation of signaling through Rho GTPases. The encoded protein inhibits the disassociation of Rho family members from GDP (guanine diphosphate), thereby maintaining these factors in an inactive state. Activity of this protein is important in a variety of cellular processes, and expression of this gene may be altered in tumors. Mutations in this gene have been found in individuals with nephrotic syndrome, type 8. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]
UniProt Code:P52565
NCBI GenInfo Identifier:1707892
NCBI Gene ID:396
NCBI Accession:P52565.3
UniProt Secondary Accession:P52565,Q6IBM5, A8MXW0, B2R5X1, B4DDD3, B4DUV9,
UniProt Related Accession:P52565
Molecular Weight:18,233 Da
NCBI Full Name:Rho GDP-dissociation inhibitor 1
NCBI Synonym Full Names:Rho GDP dissociation inhibitor alpha
NCBI Official Symbol:ARHGDIA  
NCBI Official Synonym Symbols:GDIA1; NPHS8; RHOGDI; RHOGDI-1; HEL-S-47e  
NCBI Protein Information:rho GDP-dissociation inhibitor 1
UniProt Protein Name:Rho GDP-dissociation inhibitor 1
UniProt Synonym Protein Names:Rho-GDI alpha
UniProt Gene Name:ARHGDIA  
UniProt Entry Name:GDIR1_HUMAN
Anti-ARHGDIA Antibody [KO Validated] (CAB1214)Western blot analysis of extracts from normal (control) and ARHGDIA knockout (KO) 293T cells, using ARHGDIA antibody (CAB1214) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-ARHGDIA Antibody [KO Validated] (CAB1214)Immunofluorescence analysis of HeLa cells using ARHGDIA antibody (CAB1214). Blue: DAPI for nuclear staining.
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