Cell Biology Antibodies 8
Anti-ARHGAP5 Antibody (CAB3587)
(No reviews yet)
Write a Review
- SKU:
- CAB3587
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-ARHGAP5 Antibody |
| Antibody SKU: | CAB3587 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1292-1501 of human ARHGAP5 (NP_001164.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, Jurkat, 293T, HepG2, PC-12, Mouse brain, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1292-1501 of human ARHGAP5 (NP_001164.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | EGLY RVSG NKTD QDNI QKQF DQDH NINL VSME VTVN AVAG ALKA FFAD LPDP LIPY SLHP ELLE AAKI PDKT ERLH ALKE IVKK FHPV NYDV FRYV ITHL NRVS QQHK INLM TADN LSIC FWPT LMRP DFEN REFL STTK IHQS VVET FIQQ CQFF FYNG EIVE TTNI VAPP PPSN PGQL VEPM VPLQ LPPP LQPQ LIQP QLQT DPLG II |
| Gene ID: | 394 |
| Uniprot: | Q13017 |
| Cellular Location: | Cytoplasm, Membrane, Peripheral membrane protein |
| Calculated MW: | 26kDa/27kDa/172kDa |
| Observed MW: | 185kDa |
| Synonyms: | ARHGAP5, GFI2, RhoGAP5, p190-B, p190BRhoGAP |
| Background: | Rho GTPase activating protein 5 negatively regulates RHO GTPases, a family which may mediate cytoskeleton changes by stimulating the hydrolysis of bound GTP. Two transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | ARHGAP5: a GTPase activating protein for Rho family members. May play a role in the reduction of the p21rasGTPase-activating potential of p120GAP. Negatively regulates RHO GTPases, a family which may mediate cytoskeleton changes by stimulating the hydrolysis of bound GTP. |
| UniProt Protein Details: | Protein type:GAPs; Motility/polarity/chemotaxis; GAPs, Rac/Rho Chromosomal Location of Human Ortholog: 14q12 Cellular Component: membrane; cytoplasm; cytosol Molecular Function:GTPase activity; protein binding; GTP binding; SH2 domain binding Biological Process: regulation of small GTPase mediated signal transduction; mammary gland development; metabolic process; small GTPase mediated signal transduction; positive regulation of mesenchymal cell proliferation; cell adhesion; regulation of cell size; Rho protein signal transduction; positive regulation of cell migration |
| NCBI Summary: | Rho GTPase activating protein 5 negatively regulates RHO GTPases, a family which may mediate cytoskeleton changes by stimulating the hydrolysis of bound GTP. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q13017 |
| NCBI GenInfo Identifier: | 190358871 |
| NCBI Gene ID: | 394 |
| NCBI Accession: | Q13017.2 |
| UniProt Related Accession: | Q13017 |
| Molecular Weight: | |
| NCBI Full Name: | Rho GTPase-activating protein 5 |
| NCBI Synonym Full Names: | Rho GTPase activating protein 5 |
| NCBI Official Symbol: | ARHGAP5 |
| NCBI Official Synonym Symbols: | GFI2; p190-B; RhoGAP5; p190BRhoGAP |
| NCBI Protein Information: | rho GTPase-activating protein 5 |
| UniProt Protein Name: | Rho GTPase-activating protein 5 |
| UniProt Synonym Protein Names: | Rho-type GTPase-activating protein 5; p190-B |
| UniProt Gene Name: | ARHGAP5 |
| UniProt Entry Name: | RHG05_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ARHGAP5 antibody (CAB3587) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Immunohistochemistry of paraffin-embedded rat brain using ARHGAP5 antibody (CAB3587) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of HeLa cells using ARHGAP5 antibody (CAB3587) at dilution of 1:100. Blue: DAPI for nuclear staining. |
View AllClose
Related Products
Human anti-EPO antibody(anti-Erythropoietin antibody) ELISA Kit
system_update_altDatasheet - तकनीकी मैनुअलsystem_update_altMSDS - तकनीक
Human Anti-DSG3 antibody(anti-Desmoglein-3 antibody) ELISA Kit
system_update_altDatasheet - तकनीकी मैनुअलsystem_update_altMSDS - तकनीक
Human anti-ASGPR antibody(anti asialoglyco protein receptor antibody)ELISA Kit
OverviewProduct Name:Human anti-ASGPR antibody(anti asialoglyco protein receptor antibody)ELISA KitProduct Code:AEFI00360Size:96TAlias:a