Cell Biology Antibodies 1
Anti-ARHGAP45 Antibody (CAB10454)
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- SKU:
- CAB10454
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-ARHGAP45 Antibody |
| Antibody SKU: | CAB10454 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human ARHGAP45 (NP_036424.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Rat |
| Positive Samples: | U-251MG, LO2, DU145 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human ARHGAP45 (NP_036424.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MFSR KKRE LMKT PSIS KKNR AGSP SPQP SGEL PRKD GADA VFPG PSLE PPAG SSGV KATG TLKR PTSL SRHA SAAG FPLS GAAS WTLG RSHR SPLT AASP GELP TEGA GPDV VEDI SHLL ADVA RFAE GLEK LKEC VLRD DLLE ARRP RAHE CLGE ALRV |
| Gene ID: | 23526 |
| Uniprot: | Q92619 |
| Cellular Location: | |
| Calculated MW: | 124kDa/126kDa |
| Observed MW: | 120kDa |
| Synonyms: | ARHGAP45, HA-1, HLA-HA1, HMHA1 |
| Background: |
| UniProt Protein Function: | HMHA1: GTPase activator for the Rho-type GTPases (Potential). |
| UniProt Protein Details: | Protein type:GAPs, Rac/Rho; GAPs; Cell surface Chromosomal Location of Human Ortholog: 19p13.3 Cellular Component: cytosol; membrane Molecular Function:GTPase activator activity; protein binding Biological Process: regulation of small GTPase mediated signal transduction |
| UniProt Code: | Q92619 |
| NCBI GenInfo Identifier: | 187471158 |
| NCBI Gene ID: | 23526 |
| NCBI Accession: | Q92619.2 |
| UniProt Secondary Accession: | Q92619,Q6P189, Q7LE26, Q86WS1, Q8HX84, Q9GJN9, Q9GJP0 Q9GJP1, Q9MY24, B4DTS4, F6QP70, |
| UniProt Related Accession: | Q92619 |
| Molecular Weight: | 126,497 Da |
| NCBI Full Name: | Minor histocompatibility protein HA-1 |
| NCBI Synonym Full Names: | Rho GTPase activating protein 45 |
| NCBI Official Symbol: | ARHGAP45 |
| NCBI Official Synonym Symbols: | HA-1; HMHA1; HLA-HA1 |
| NCBI Protein Information: | minor histocompatibility protein HA-1 |
| UniProt Protein Name: | Minor histocompatibility protein HA-1 |
| Protein Family: | Minor histocompatibility protein |
| UniProt Gene Name: | HMHA1 |
| UniProt Entry Name: | HMHA1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ARHGAP45 antibody (CAB10454) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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