Cell Biology Antibodies 8
Anti-ARHGAP1 Antibody (CAB3739)
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- SKU:
- CAB3739
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-ARHGAP1 Antibody |
| Antibody SKU: | CAB3739 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF IP |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 210-439 of human ARHGAP1 (NP_004299.1). |
| Application: | WB IF IP |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 IP 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | BxPC-3, HeLa, HepG2, U-87MG, PC-12, Mouse lung |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 210-439 of human ARHGAP1 (NP_004299.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | VLKY DDFL KSTQ KSPA TAPK PMPP RPPL PNQQ FGVS LQHL QEKN PEQE PIPI VLRE TVAY LQAH ALTT EGIF RRSA NTQV VREV QQKY NMGL PVDF DQYN ELHL PAVI LKTF LREL PEPL LTFD LYPH VVGF LNID ESQR VPAT LQVL QTLP EENY QVLR FLTA FLVQ ISAH SDQN KMTN TNLA VVFG PNLL WAKD AAIT LKAI NPIN TFTK FLLD HQGE LFPS PDPS GL |
| Gene ID: | 392 |
| Uniprot: | Q07960 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 50kDa |
| Observed MW: | 50kDa |
| Synonyms: | ARHGAP1, CDC42GAP, RHOGAP, RHOGAP1, p50rhoGAP |
| Background: | This gene encodes a member of a large family of proteins that activate Rho-type guanosine triphosphate (GTP) metabolizing enzymes. The encoded protein contains a SRC homology 3 domain and interacts with Bcl-2-associated protein family members. |
| UniProt Protein Function: | ARHGAP1: GTPase activator for the Rho, Rac and Cdc42 proteins, converting them to the putatively inactive GDP-bound state. Cdc42 seems to be the preferred substrate. |
| UniProt Protein Details: | Protein type:GAPs; Motility/polarity/chemotaxis; GAPs, Rac/Rho Chromosomal Location of Human Ortholog: 11p11.2 Cellular Component: ruffle; plasma membrane; cytosol Molecular Function:protein binding; SH3/SH2 adaptor activity; SH3 domain binding Biological Process: regulation of small GTPase mediated signal transduction; positive regulation of signal transduction; small GTPase mediated signal transduction; Rho protein signal transduction |
| NCBI Summary: | This gene encodes a member of a large family of proteins that activate Rho-type guanosine triphosphate (GTP) metabolizing enzymes. The encoded protein contains a SRC homology 3 domain and interacts with Bcl-2-associated protein family members. [provided by RefSeq, Aug 2012] |
| UniProt Code: | Q07960 |
| NCBI GenInfo Identifier: | 3024550 |
| NCBI Gene ID: | 392 |
| NCBI Accession: | Q07960.1 |
| UniProt Secondary Accession: | Q07960,D3DQQ6, |
| UniProt Related Accession: | Q07960 |
| Molecular Weight: | 439 |
| NCBI Full Name: | Rho GTPase-activating protein 1 |
| NCBI Synonym Full Names: | Rho GTPase activating protein 1 |
| NCBI Official Symbol: | ARHGAP1 |
| NCBI Official Synonym Symbols: | RHOGAP; RHOGAP1; CDC42GAP; p50rhoGAP |
| NCBI Protein Information: | rho GTPase-activating protein 1; CDC42 GTPase-activating protein; GTPase-activating protein rhoGAP; GTPase-activating protein rhoOGAP; GTPase-activating protein, Rho, 1; rho-type GTPase-activating protein 1; rho-related small GTPase protein activator |
| UniProt Protein Name: | Rho GTPase-activating protein 1 |
| UniProt Synonym Protein Names: | CDC42 GTPase-activating protein; GTPase-activating protein rhoOGAP; Rho-related small GTPase protein activator; Rho-type GTPase-activating protein 1; p50-RhoGAP |
| Protein Family: | Rho GTPase-activating protein |
| UniProt Gene Name: | ARHGAP1 |
| UniProt Entry Name: | RHG01_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ARHGAP1 antibody (CAB3739) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s. |
 | Immunofluorescence analysis of HeLa cells using ARHGAP1 antibody (CAB3739) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug ARHGAP1 antibody (CAB3739). Western blot was performed from the immunoprecipitate using ARHGAP1 antibody (CAB3739) at a dilition of 1:1000. |
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