Immunology Antibodies 2
Anti-ARG1 / Arginase 1 Antibody (CAB1847)
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- SKU:
- CAB1847
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Immunology
Description
| Antibody Name: | Anti-ARG1 / Arginase 1 Antibody |
| Antibody SKU: | CAB1847 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-322 of human ARG1 / Arginase 1 (NP_000036.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HepG2, Rat liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-322 of human ARG1 / Arginase 1 (NP_000036.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MSAK SRTI GIIG APFS KGQP RGGV EEGP TVLR KAGL LEKL KEQE CDVK DYGD LPFA DIPN DSPF QIVK NPRS VGKA SEQL AGKV AEVK KNGR ISLV LGGD HSLA IGSI SGHA RVHP DLGV IWVD AHTD INTP LTTT SGNL HGQP VSFL LKEL KGKI PDVP GFSW VTPC ISAK DIVY IGLR DVDP GEHY ILKT LGIK YFSM TEVD RLGI GKVM EETL SYLL GRKK RPIH LSFD VDGL DPSF TPAT GTPV VGGL TYRE GLYI TEEI YKTG LLSG LDIM EVNP SLGK TPEE VTRT VNTA VAIT LACF GLAR EGNH KPID YLNP PK |
| Gene ID: | 383 |
| Uniprot: | P05089 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 25kDa/34kDa/35kDa |
| Observed MW: | 40KDa |
| Synonyms: | ARG1, arginase-1 |
| Background: | Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform encoded by this gene, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. Two transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | ARG1: Homotrimer. By arginine or homoarginine. Belongs to the arginase family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Amino Acid Metabolism - arginine and proline; EC 3.5.3.1; Hydrolase Chromosomal Location of Human Ortholog: 6q23.2 Cellular Component: cytoplasm; cytosol; extracellular region; nucleus Molecular Function:arginase activity Biological Process: arginine catabolic process; neutrophil degranulation; urea cycle Disease: Argininemia |
| NCBI Summary: | Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform encoded by this gene, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011] |
| UniProt Code: | P05089 |
| NCBI GenInfo Identifier: | 12230985 |
| NCBI Gene ID: | 383 |
| NCBI Accession: | P05089.2 |
| UniProt Secondary Accession: | P05089,Q5JWT5, Q5JWT6, Q8TE72, Q9BS50, A6NEA0, |
| UniProt Related Accession: | P05089 |
| Molecular Weight: | 35kDa |
| NCBI Full Name: | Arginase-1 |
| NCBI Synonym Full Names: | arginase 1 |
| NCBI Official Symbol: | ARG1 |
| NCBI Protein Information: | arginase-1 |
| UniProt Protein Name: | Arginase-1 |
| UniProt Synonym Protein Names: | Liver-type arginase; Type I arginase |
| Protein Family: | Arginase |
| UniProt Gene Name: | ARG1 |
 | Western blot analysis of extracts of Rat liver, using ARG1 / Arginase 1 Rabbit pAb (CAB1847) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Western blot analysis of extracts of HepG2 cells, using ARG1 / Arginase 1 Rabbit pAb (CAB1847) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunohistochemistry of paraffin-embedded mouse liver using ARG1 / Arginase 1 Rabbit pAb (CAB1847) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of Rat liver using ARG1 / Arginase 1 antibody (CAB1847) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of Human liver using ARG1 / Arginase 1 antibody (CAB1847) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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