Anti-ARFRP1 / ARP Antibody (CAB20884)
- SKU:
- CAB20884
- Product Type:
- Antibody
- Antibody Type:
- Polyclonal Antibody
- Reactivity:
- Plant
- Host Species:
- Rabbit
- Isotype:
- IgG
Description
| Antibody Name: | Anti-ARFRP1 / ARP Antibody (CAB20884) |
| Antibody SKU: | CAB20884 |
| Antibody Size: | 50µL, 100µL |
| Application: | Western blotting, Immunofluorescence |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant protein of Human ARFRP1 / ARP . |
| Application: | Western blotting, Immunofluorescence |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HepG2, PC-3, HeLa, Mouse liver, Mouse heart, Rat lung |
| Immunogen: | Recombinant protein of Human ARFRP1 / ARP . |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Cellular Location: | Golgi apparatus, trans-Golgi network |
| Calculated MW: | 13kDa/17kDa/19kDa/22kDa |
| Observed MW: | 25KDa |
| Synonyms: | ARFRP1, ARL18, ARP, Arp1 |
| Background: | The protein encoded by this gene is a membrane-associated GTP-ase which localizes to the plasma membrane and is related to the ADP-ribosylation factor (ARF) and ARF-like (ARL) proteins. This gene plays a role in membrane trafficking between the trans-Golgi network and endosomes. Alternatively spliced transcript variants encoding different isoforms have been identified. |
 | Western blot analysis of extracts of various cell lines, using ARFRP1/ARP antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s. |
 | Immunofluorescence analysis of Jurkat cells using ARFRP1 / ARP Rabbit mAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining. |
 | Western blot analysis of extracts of various cell lines, using ARFRP1/ARP antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s. |
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