Name: Anti-ARFRP1 Antibody (CAB14311)
Brand: Cell Biology Antibodies 4
SKU: CAB14311
Availability: OutOfStock

Antibody Name:	Anti-ARFRP1 Antibody
Antibody SKU:	CAB14311
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-201 of human ARFRP1 (NP_001254476.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG, 293T, Mouse brain, Mouse heart, Rat lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-201 of human ARFRP1 (NP_001254476.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MYTL LSGL YKYM FQKD EYCI LILG LDNA GKTT FLEQ SKTR FNKN YKGM SLSK ITTT VGLN IGTV DVGK ARLM FWDL GGQE ELQS LWDK YYAE CHGV IYVI DSTD EERL AESK QAFE KVVT SEAL CGVP VLVL ANKQ DVET CLSI PDIK TAFS DCTS KIGR RDCL TQAC SALT GKGV REGI EWMV KCVV RNVH RPPR QRDI T
Gene ID:	10139
Uniprot:	Q13795
Cellular Location:	Golgi apparatus, trans-Golgi network
Calculated MW:	13kDa/17kDa/19kDa/22kDa
Observed MW:	25kDa

Synonyms:	ARFRP1, ARL18, ARP, Arp1
Background:	The protein encoded by this gene is a membrane-associated GTP-ase which localizes to the plasma membrane and is related to the ADP-ribosylation factor (ARF) and ARF-like (ARL) proteins. This gene plays a role in membrane trafficking between the trans-Golgi network and endosomes. Alternatively spliced transcript variants encoding different isoforms have been identified.

UniProt Protein Function:	AGO4: Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Also required for RNA-directed transcription and replication of the human hapatitis delta virus (HDV). Interacts with EIF4B, IMP8, PRMT5, TNRC6A and TNRC6B. Belongs to the argonaute family. Ago subfamily.
UniProt Protein Details:	Protein type:Translation; Translation initiation Chromosomal Location of Human Ortholog: 1p34 Cellular Component: cytoplasm; cytosol; membrane Molecular Function:double-stranded RNA binding; miRNA binding; protein binding; single-stranded RNA binding Biological Process: miRNA-mediated gene silencing, miRNA loading onto RISC; miRNA-mediated gene silencing, mRNA cleavage; miRNA-mediated gene silencing, negative regulation of translation; miRNA-mediated gene silencing, production of miRNAs; mRNA catabolic process; phosphoinositide-mediated signaling; pre-microRNA processing; RNA secondary structure unwinding; RNA-mediated posttranscriptional gene silencing; Wnt receptor signaling pathway, calcium modulating pathway
NCBI Summary:	The protein encoded by this gene is a membrane-associated GTP-ase which localizes to the plasma membrane and is related to the ADP-ribosylation factor (ARF) and ARF-like (ARL) proteins. This gene plays a role in membrane trafficking between the trans-Golgi network and endosomes. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, May 2012]
UniProt Code:	Q13795
NCBI GenInfo Identifier:	4507449
NCBI Gene ID:	10139
NCBI Accession:	NP_003215
UniProt Related Accession:	Q13795
Molecular Weight:	97
NCBI Full Name:	ADP-ribosylation factor-related protein 1 isoform a
NCBI Synonym Full Names:	ADP ribosylation factor related protein 1
NCBI Official Symbol:	ARFRP1
NCBI Official Synonym Symbols:	ARP; Arp1; ARL18
NCBI Protein Information:	ADP-ribosylation factor-related protein 1
UniProt Protein Name:	Protein argonaute-4
UniProt Synonym Protein Names:	Argonaute RISC catalytic component 4; Eukaryotic translation initiation factor 2C 4
Protein Family:	Protein argonaute
UniProt Gene Name:	AGO4
UniProt Entry Name:	AGO4_HUMAN

Western blot analysis of extracts of various cell lines, using ARFRP1 antibody (CAB14311) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Antibodies

ARFRP1 Antibody (PACO07814)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)