KO Validated Antibodies 2

Anti-ARF5 Antibody (CAB19986)[KO Validated]

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SKU:
CAB19986
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-ARF5 Antibody [KO Validated]
Antibody SKU:CAB19986
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant protein of human ARF5.
Application:WB IF
Recommended Dilution:WB 1:1000 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:293T
Immunogen:Recombinant protein of human ARF5.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:381
Uniprot:P84085
Cellular Location:Cytoplasm, Golgi apparatus, Lipid-anchor, Membrane, perinuclear region
Calculated MW:20kDa
Observed MW:18KDa
Anti-ARF5 Antibody [KO Validated] (CAB19986)Western blot analysis of extracts from normal (control) and ARF5 knockout (KO) 293T cells, using ARF5 antibody (CAB19986) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
Synonyms:ARF5
Background:This gene is a member of the human ADP-ribosylation factor (ARF) gene family. These genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 6 ARF proteins and 11 ARF-like proteins and constitute 1 family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2, and ARF3), class II (ARF4 and ARF5) and class III (ARF6). The members of each class share a common gene organization.
UniProt Protein Function:ARF5: GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP- ribosyltransferase. Involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus. Belongs to the small GTPase superfamily. Arf family.
UniProt Protein Details:

Protein type:G protein, monomeric, ARF; G protein; G protein, monomeric; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 7q31.3

Cellular Component: Golgi apparatus; perinuclear region of cytoplasm; plasma membrane

Molecular Function:GTPase activity; protein binding; GTP binding

Biological Process: vesicle-mediated transport; protein transport; metabolic process; small GTPase mediated signal transduction

NCBI Summary:This gene is a member of the human ADP-ribosylation factor (ARF) gene family. These genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 6 ARF proteins and 11 ARF-like proteins and constitute 1 family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2,and ARF3), class II (ARF4 and ARF5) and class III (ARF6). The members of each class share a common gene organization. [provided by RefSeq, Dec 2010]
UniProt Code:P84085
NCBI GenInfo Identifier:51316990
NCBI Gene ID:381
NCBI Accession:P84085.2
UniProt Secondary Accession:P84085,P26437,
UniProt Related Accession:P84085
Molecular Weight:180
NCBI Full Name:ADP-ribosylation factor 5
NCBI Synonym Full Names:ADP-ribosylation factor 5
NCBI Official Symbol:ARF5  
NCBI Protein Information:ADP-ribosylation factor 5
UniProt Protein Name:ADP-ribosylation factor 5
Protein Family:Auxin response factor
UniProt Gene Name:ARF5  
UniProt Entry Name:ARF5_HUMAN
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