Cell Biology Antibodies 11

Anti-ARF4 Antibody (CAB7644)

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SKU:
CAB7644
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-ARF4 Antibody
Antibody SKU:CAB7644
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human ARF4 (NP_001651.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:SH-SY5Y, Mouse brain, Mouse testis, Rat testis, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human ARF4 (NP_001651.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MGLT ISSL FSRL FGKK QMRI LMVG LDAA GKTT ILYK LKLG EIVT TIPT IGFN VETV EYKN ICFT VWDV GGQD RIRP LWKH YFQN TQGL IFVV DSND RERI QEVA DELQ KMLL VDEL RDAV LLLF ANKQ DLPN AMAI SEMT DKLG LQSL RNRT WYVQ ATCA TQGT GLYE GLDW LSNE LSKR
Gene ID:378
Uniprot:P18085
Cellular Location:Golgi apparatus, Lipid-anchor, Membrane
Calculated MW:20kDa
Observed MW:14kDa
Synonyms:ARF4, ARF2
Background:This gene is a member of the human ARF gene family whose members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 5 ARF proteins and 11 ARF-like proteins and constitute one family of the RAS superfamily. The ARF proteins are categorized as class I, class II and class III; this gene is a class II member. The members of each class share a common gene organization. The ARF4 gene spans approximately 12kb and contains six exons and five introns. This gene is the most divergent member of the human ARFs. Conflicting map positions at 3p14 or 3p21 have been reported for this gene.
UniProt Protein Function:ARF4: GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP- ribosyltransferase. Involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus. Belongs to the small GTPase superfamily. Arf family.
UniProt Protein Details:

Protein type:G protein, monomeric; Motility/polarity/chemotaxis; G protein, monomeric, ARF; G protein

Chromosomal Location of Human Ortholog: 3p14.3

Cellular Component: cytosol; dendritic spine; membrane

Molecular Function:ARF guanyl-nucleotide exchange factor activity; epidermal growth factor receptor binding; GTP binding; GTPase activity; protein binding

Biological Process: cell migration; ER to Golgi vesicle-mediated transport; negative regulation of apoptosis; phospholipase D activation; positive regulation of transcription from RNA polymerase II promoter; protein amino acid ADP-ribosylation; retrograde vesicle-mediated transport, Golgi to ER

NCBI Summary:This gene is a member of the human ARF gene family whose members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 5 ARF proteins and 11 ARF-like proteins and constitute one family of the RAS superfamily. The ARF proteins are categorized as class I, class II and class III; this gene is a class II member. The members of each class share a common gene organization. The ARF4 gene spans approximately 12kb and contains six exons and five introns. This gene is the most divergent member of the human ARFs. Conflicting map positions at 3p14 or 3p21 have been reported for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:P18085
NCBI GenInfo Identifier:114123
NCBI Gene ID:378
NCBI Accession:P18085.3
UniProt Secondary Accession:P18085,P21371, B2R7J7,
UniProt Related Accession:P18085
Molecular Weight:20511 Da
NCBI Full Name:ADP-ribosylation factor 4
NCBI Synonym Full Names:ADP ribosylation factor 4
NCBI Official Symbol:ARF4  
NCBI Official Synonym Symbols:ARF2  
NCBI Protein Information:ADP-ribosylation factor 4
UniProt Protein Name:ADP-ribosylation factor 4
Protein Family:Auxin response factor
UniProt Gene Name:ARF4  
UniProt Entry Name:ARF4_HUMAN
Anti-ARF4 Antibody (CAB7644)Western blot analysis of extracts of various cell lines, using ARF4 antibody (CAB7644) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-ARF4 Antibody (CAB7644)Immunofluorescence analysis of C6 cells using ARF4 Rabbit pAb (CAB7644) at dilution of 1:100. Blue: DAPI for nuclear staining.
Anti-ARF4 Antibody (CAB7644)Immunofluorescence analysis of NIH/3T3 cells using ARF4 Rabbit pAb (CAB7644) at dilution of 1:100. Blue: DAPI for nuclear staining.
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