Cell Biology Antibodies 11

Anti-ARF1 Antibody (CAB7643)

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SKU:
CAB7643
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-ARF1 Antibody
Antibody SKU:CAB7643
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-181 of human ARF1 (NP_001649.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:SH-SY5Y, 293T, BT-474, THP-1, Mouse kidney, Rat kidney, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-181 of human ARF1 (NP_001649.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MGNI FANL FKGL FGKK EMRI LMVG LDAA GKTT ILYK LKLG EIVT TIPT IGFN VETV EYKN ISFT VWDV GGQD KIRP LWRH YFQN TQGL IFVV DSND RERV NEAR EELM RMLA EDEL RDAV LLVF ANKQ DLPN AMNA AEIT DKLG LHSL RHRN WYIQ ATCA TSGD GLYE GLDW LSNQ LRNQ K
Gene ID:375
Uniprot:P84077
Cellular Location:Cell junction, Cytoplasm, Golgi apparatus, Lipid-anchor, Membrane, perinuclear region, postsynaptic cell membrane, postsynaptic density, synapse, synaptosome
Calculated MW:20kDa
Observed MW:18kDa
Synonyms:ARF1
Background:ADP-ribosylation factor 1 (ARF1) is a member of the human ARF gene family. The family members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking as activators of phospholipase D. The gene products, including 6 ARF proteins and 11 ARF-like proteins, constitute a family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2 and ARF3), class II (ARF4 and ARF5) and class III (ARF6), and members of each class share a common gene organization. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.
UniProt Protein Function:ARF1: GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP- ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles. Belongs to the small GTPase superfamily. Arf family.
UniProt Protein Details:

Protein type:G protein; G protein, monomeric, ARF; Motility/polarity/chemotaxis; G protein, monomeric

Chromosomal Location of Human Ortholog: 1q42

Cellular Component: Golgi membrane; postsynaptic membrane; neuron projection; sarcomere; focal adhesion; perinuclear region of cytoplasm; postsynaptic density; plasma membrane; cytosol

Molecular Function:GTPase activity; protein binding; GTP binding; receptor signaling protein activity

Biological Process: protein transport; cellular copper ion homeostasis; COPI coating of Golgi vesicle; viral reproduction; regulation of receptor internalization; small GTPase mediated signal transduction; phospholipid metabolic process; phosphatidylinositol biosynthetic process; retrograde vesicle-mediated transport, Golgi to ER; antigen processing and presentation of exogenous peptide antigen via MHC class II; post-Golgi vesicle-mediated transport; regulation of defense response to virus by virus

NCBI Summary:ADP-ribosylation factor 1 (ARF1) is a member of the human ARF gene family. The family members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking as activators of phospholipase D. The gene products, including 6 ARF proteins and 11 ARF-like proteins, constitute a family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2 and ARF3), class II (ARF4 and ARF5) and class III (ARF6), and members of each class share a common gene organization. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:P84077
NCBI GenInfo Identifier:51316985
NCBI Gene ID:375
NCBI Accession:P84077.2
UniProt Secondary Accession:P84077,P10947, P32889,
UniProt Related Accession:P84077
Molecular Weight:181
NCBI Full Name:ADP-ribosylation factor 1
NCBI Synonym Full Names:ADP-ribosylation factor 1
NCBI Official Symbol:ARF1  
NCBI Protein Information:ADP-ribosylation factor 1
UniProt Protein Name:ADP-ribosylation factor 1
Protein Family:Auxin response factor
UniProt Gene Name:ARF1  
UniProt Entry Name:ARF1_HUMAN
Anti-ARF1 Antibody (CAB7643)Western blot analysis of extracts of various cell lines, using ARF1 antibody (CAB7643) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s.
Anti-ARF1 Antibody (CAB7643)Immunofluorescence analysis of HeLa cells using ARF1 antibody (CAB7643) at dilution of 1:100. Blue: DAPI for nuclear staining.
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