Antibody Name:	Anti-ARF1 Antibody
Antibody SKU:	CAB2821
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide of human ARF1

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	BT-474, HT-29, SKOV3, MCF7, HeLa, Mouse lung, Mouse brain, Mouse heart, Rat liver

Immunogen:	A synthetic peptide of human ARF1
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	375
Uniprot:	P84077
Cellular Location:	Cell junction, Cytoplasm, Golgi apparatus, Lipid-anchor, Membrane, perinuclear region, postsynaptic cell membrane, postsynaptic density, synapse, synaptosome
Calculated MW:	20kDa
Observed MW:	17kDa

Synonyms:

ARF1

Background:

ADP-ribosylation factor 1 (ARF1) is a member of the human ARF gene family. The family members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking as activators of phospholipase D. The gene products, including 6 ARF proteins and 11 ARF-like proteins, constitute a family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2 and ARF3), class II (ARF4 and ARF5) and class III (ARF6), and members of each class share a common gene organization. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.

UniProt Protein Function:	ARF1: GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP- ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles. Belongs to the small GTPase superfamily. Arf family.
UniProt Protein Details:	Protein type:G protein; G protein, monomeric, ARF; Motility/polarity/chemotaxis; G protein, monomeric Chromosomal Location of Human Ortholog: 1q42 Cellular Component: Golgi membrane; postsynaptic membrane; neuron projection; sarcomere; focal adhesion; perinuclear region of cytoplasm; postsynaptic density; plasma membrane; cytosol Molecular Function:GTPase activity; protein binding; GTP binding; receptor signaling protein activity Biological Process: protein transport; cellular copper ion homeostasis; COPI coating of Golgi vesicle; viral reproduction; regulation of receptor internalization; small GTPase mediated signal transduction; phospholipid metabolic process; phosphatidylinositol biosynthetic process; retrograde vesicle-mediated transport, Golgi to ER; antigen processing and presentation of exogenous peptide antigen via MHC class II; post-Golgi vesicle-mediated transport; regulation of defense response to virus by virus
NCBI Summary:	ADP-ribosylation factor 1 (ARF1) is a member of the human ARF gene family. The family members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking as activators of phospholipase D. The gene products, including 6 ARF proteins and 11 ARF-like proteins, constitute a family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2 and ARF3), class II (ARF4 and ARF5) and class III (ARF6), and members of each class share a common gene organization. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	P84077
NCBI GenInfo Identifier:	51316985
NCBI Gene ID:	375
NCBI Accession:	P84077.2
UniProt Secondary Accession:	P84077,P10947, P32889,
UniProt Related Accession:	P84077
Molecular Weight:	181
NCBI Full Name:	ADP-ribosylation factor 1
NCBI Synonym Full Names:	ADP-ribosylation factor 1
NCBI Official Symbol:	ARF1
NCBI Protein Information:	ADP-ribosylation factor 1
UniProt Protein Name:	ADP-ribosylation factor 1
Protein Family:	Auxin response factor
UniProt Gene Name:	ARF1
UniProt Entry Name:	ARF1_HUMAN

	Western blot analysis of extracts of various cell lines, using ARF1 antibody (CAB2821) at 1:500 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 60s.
	Immunohistochemistry of paraffin-embedded mouse brain using ARF1 antibody (CAB2821) (40x lens).
	Immunohistochemistry of paraffin-embedded rat kidney using ARF1 antibody (CAB2821) (40x lens).
	Immunohistochemistry of paraffin-embedded human prostate using ARF1 antibody (CAB2821) (40x lens).
	Immunofluorescence analysis of HeLa cells using ARF1 antibody (CAB2821) at dilution of 1:100. Blue: DAPI for nuclear staining.