Cell Biology Antibodies 5

Anti-ARCN1 Antibody (CAB14993)

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SKU:
CAB14993
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-ARCN1 Antibody
Antibody SKU:CAB14993
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 212-511 of human ARCN1 (NP_001646.2).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:LO2, HeLa, NIH/3T3, Mouse brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 212-511 of human ARCN1 (NP_001646.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:VAPA PARP SGPS KALK LGAK GKEV DNFV DKLK SEGE TIMS SSMG KRTS EATK MHAP PINM ESVH MKIE EKIT LTCG RDGG LQNM ELHG MIML RISD DKYG RIRL HVEN EDKK GVQL QTHP NVDK KLFT AESL IGLK NPEK SFPV NSDV GVLK WRLQ TTEE SFIP LTIN CWPS ESGN GCDV NIEY ELQE DNLE LNDV VITI PLPS GVGA PVIG EIDG EYRH DSRR NTLE WCLP VIDA KNKS GSLE FSIA GQPN DFFP VQVS FVSK KNYC NIQV TKVT QVDG NSPV RFST ETTF LVDK YEIL
Gene ID:372
Uniprot:P48444
Cellular Location:COPI-coated vesicle membrane, Cytoplasm, Cytoplasmic side, Cytoplasmic vesicle, Golgi apparatus membrane, Peripheral membrane protein
Calculated MW:47kDa/57kDa
Observed MW:65kDa
Synonyms:ARCN1, COPD, SRMMD, archain 1
Background:This gene maps in a region, which include the mixed lineage leukemia and Friend leukemia virus integration 1 genes, where multiple disease-associated chromosome translocations occur. It is an intracellular protein. Archain sequences are well conserved among eukaryotes and this protein may play a fundamental role in eukaryotic cell biology. It has similarities to heat shock proteins and clathrin-associated proteins, and may be involved in vesicle structure or trafficking.
UniProt Protein Function:ARCN1: The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors. Belongs to the adaptor complexes medium subunit family. Delta-COP subfamily.
UniProt Protein Details:

Protein type:Vesicle

Chromosomal Location of Human Ortholog: 11q23.3

Cellular Component: COPI vesicle coat; cytoplasm; cytosol; endoplasmic reticulum membrane; Golgi apparatus; Golgi membrane; intracellular membrane-bound organelle; membrane; transport vesicle

Biological Process: ER to Golgi vesicle-mediated transport; intracellular protein transport; retrograde vesicle-mediated transport, Golgi to ER

NCBI Summary:This gene maps in a region, which include the mixed lineage leukemia and Friend leukemia virus integration 1 genes, where multiple disease-associated chromosome translocations occur. It is an intracellular protein. Archain sequences are well conserved among eukaryotes and this protein may play a fundamental role in eukaryotic cell biology. It has similarities to heat shock proteins and clathrin-associated proteins, and may be involved in vesicle structure or trafficking. [provided by RefSeq, Jul 2008]
UniProt Code:P48444
NCBI GenInfo Identifier:1351970
NCBI Gene ID:372
NCBI Accession:P48444.1
UniProt Secondary Accession:P48444,Q52M80, B4E1X2, E9PEU4,
UniProt Related Accession:P48444
Molecular Weight:47,205 Da
NCBI Full Name:Coatomer subunit delta
NCBI Synonym Full Names:archain 1
NCBI Official Symbol:ARCN1  
NCBI Official Synonym Symbols:COPD  
NCBI Protein Information:coatomer subunit delta
UniProt Protein Name:Coatomer subunit delta
UniProt Synonym Protein Names:Archain; Delta-coat protein; Delta-COP
Protein Family:Coatomer
UniProt Gene Name:ARCN1  
UniProt Entry Name:COPD_HUMAN
Anti-ARCN1 Antibody (CAB14993)Western blot analysis of extracts of various cell lines, using ARCN1 antibody (CAB14993) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
Anti-ARCN1 Antibody (CAB14993)Immunofluorescence analysis of NIH/3T3 cells using ARCN1 Rabbit pAb (CAB14993) at dilution of 1:100. Blue: DAPI for nuclear staining.
Anti-ARCN1 Antibody (CAB14993)Immunofluorescence analysis of HeLa cells using ARCN1 Rabbit pAb (CAB14993) at dilution of 1:100. Blue: DAPI for nuclear staining.
Anti-ARCN1 Antibody (CAB14993)Immunofluorescence analysis of C6 cells using ARCN1 Rabbit pAb (CAB14993) at dilution of 1:100. Blue: DAPI for nuclear staining.
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