Cell Biology Antibodies 2

Anti-ARAP2 Antibody (CAB12254)

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SKU:
CAB12254
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-ARAP2 Antibody
Antibody SKU:CAB12254
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1405-1704 of human ARAP2 (NP_056045.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:293T, K-562, Mouse brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1405-1704 of human ARAP2 (NP_056045.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:LAEP GSAY LVVK RFLT ADTI KHCS DRST LGSI KEGI LKIK EEPS KILS GNKF QDRY FVLR DGFL FLYK DVKS SKHD KMFS LSSM KFYR GVKK KMKP PTSW GLTA YSEK HHWH LCCD SSRT QTEW MTSI FIAQ HEYD IWPP AGKE RKRS ITKN PKIG GLPL IPIQ HEGN ATLA RKNI ESAR AELE RLRL SEKC DKES VDSS LKER ASMV AHCL EHKD DKLR NRPR KHRS FNCL EDTE PEAP LGQP KGHK GLKT LRKT EDRN SKAT LDSD HKLP SRVI EELN VVLQ RSRT LPKE LQDE QILK
Gene ID:116984
Uniprot:Q8WZ64
Cellular Location:Cytoplasm
Calculated MW:193kDa
Observed MW:170kDa
Synonyms:ARAP2, CENTD1, PARX
Background:The protein encoded by this gene contains ARF-GAP, RHO-GAP, ankyrin repeat, RAS-associating, and pleckstrin homology domains. The protein is a phosphatidylinositol (3, 4, 5)-trisphosphate-dependent Arf6 GAP that binds RhoA-GTP, but it lacks the predicted catalytic arginine in the RHO-GAP domain and does not have RHO-GAP activity. The protein associates with focal adhesions and functions downstream of RhoA to regulate focal adhesion dynamics.
UniProt Protein Function:CENTD1: Phosphatidylinositol 3,4,5-trisphosphate-dependent GTPase-activating protein that modulates actin cytoskeleton remodeling by regulating ARF and RHO family members. Is activated by phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3) binding. Can be activated by phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4,5)P2) binding, albeit with lower efficiency.
UniProt Protein Details:

Protein type:GAP; GAP, ARF

Chromosomal Location of Human Ortholog: 4p14

Cellular Component: cytosol

Molecular Function:GTPase activator activity; phosphatidylinositol-3,4,5-triphosphate binding

Biological Process: regulation of small GTPase mediated signal transduction

NCBI Summary:The protein encoded by this gene contains ARF-GAP, RHO-GAP, ankyrin repeat, RAS-associating, and pleckstrin homology domains. The protein is a phosphatidylinositol (3,4,5)-trisphosphate-dependent Arf6 GAP that binds RhoA-GTP, but it lacks the predicted catalytic arginine in the RHO-GAP domain and does not have RHO-GAP activity. The protein associates with focal adhesions and functions downstream of RhoA to regulate focal adhesion dynamics. [provided by RefSeq, Sep 2008]
UniProt Code:Q8WZ64
NCBI GenInfo Identifier:308153629
NCBI Gene ID:116984
NCBI Accession:Q8WZ64.3
UniProt Secondary Accession:Q8WZ64,Q4W5D2, Q7Z2L5, Q96L70, Q96P49, Q9Y4E4,
UniProt Related Accession:Q8WZ64
Molecular Weight:Observed MW 170kDaCalculated MW 193kDa
NCBI Full Name:Arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 2
NCBI Synonym Full Names:ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 2
NCBI Official Symbol:ARAP2  
NCBI Official Synonym Symbols:PARX; CENTD1  
NCBI Protein Information:arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 2
UniProt Protein Name:Arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 2
UniProt Synonym Protein Names:Centaurin-delta-1; Cnt-d1; Protein PARX
Protein Family:Arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein
UniProt Gene Name:ARAP2  
Anti-ARAP2 Antibody (CAB12254)Western blot analysis of extracts of various cell lines, using ARAP2 antibody (CAB12254) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
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