Cell Biology Antibodies 7

Anti-ARAF Antibody (CAB16346)

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SKU:
CAB16346
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-ARAF Antibody
Antibody SKU:CAB16346
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 371-606 of human ARAF (NP_001645.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 371-606 of human ARAF (NP_001645.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:GFMT RPGF AIIT QWCE GSSL YHHL HVAD TRFD MVQL IDVA RQTA QGMD YLHA KNII HRDL KSNN IFLH EGLT VKIG DFGL ATVK TRWS GAQP LEQP SGSV LWMA AEVI RMQD PNPY SFQS DVYA YGVV LYEL MTGS LPYS HIGC RDQI IFMV GRGY LSPD LSKI SSNC PKAM RRLL SDCL KFQR EERP LFPQ ILAT IELL QRSL PKIE RSAS EPSL HRTQ ADEL PACL LSAA RLVP
Gene ID:369
Uniprot:P10398
Cellular Location:
Calculated MW:20kDa/67kDa
Observed MW:Refer to figures
Synonyms:ARAF, A-RAF, ARAF1, PKS2, RAFA1
Background:This proto-oncogene belongs to the RAF subfamily of the Ser/Thr protein kinase family, and maybe involved in cell growth and development. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:ARAF: a tyrosine kinase-like kinase of the RAF family. Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Frequently mutated in thyroid cancers, skin melanomas and at lower frequency in a wide range of human cancers. An activating mutation, mimicking phosphorylation of the activation loop, is seen in 60% of malignant melanoma samples. Raf mutations are generally exclusive to Ras activating mutations. Activating mutations are also seen in ~10% of colorectal cancers, in lung cancers and gliomas, and at a lower rate in several other tumors. Inactivating mutations are also seen and may result in activation of c-Raf and Erk. Mutations in B-Raf, MEK1 and MEK2 also associated with cardiofaciocutaneous syndrome, displaying morphological, cardiac and mental defects. Approved Inhibitor: Nexavar/Sorafenib.
UniProt Protein Details:

Protein type:EC 2.7.11.1; Oncoprotein; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, TKL; TKL group; RAF family

Chromosomal Location of Human Ortholog: Xp11.4-p11.2

Cellular Component: cytosol

Molecular Function:protein binding; protein kinase activity; protein serine/threonine kinase activity

Biological Process: MAPKKK cascade; negative regulation of apoptosis; positive regulation of peptidyl-serine phosphorylation; protein modification process; regulation of proteasomal ubiquitin-dependent protein catabolic process; regulation of TOR signaling pathway

NCBI Summary:This proto-oncogene belongs to the RAF subfamily of the Ser/Thr protein kinase family, and maybe involved in cell growth and development. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2012]
UniProt Code:P10398
NCBI GenInfo Identifier:1730068
NCBI Gene ID:369
NCBI Accession:P10398.2
UniProt Secondary Accession:P10398,P07557, Q5H9B2, Q5H9B3,
UniProt Related Accession:P10398
Molecular Weight:20,904 Da
NCBI Full Name:Serine/threonine-protein kinase A-Raf
NCBI Synonym Full Names:A-Raf proto-oncogene, serine/threonine kinase
NCBI Official Symbol:ARAF  
NCBI Official Synonym Symbols:PKS2; A-RAF; ARAF1; RAFA1  
NCBI Protein Information:serine/threonine-protein kinase A-Raf
UniProt Protein Name:Serine/threonine-protein kinase A-Raf
UniProt Synonym Protein Names:Proto-oncogene A-Raf; Proto-oncogene A-Raf-1; Proto-oncogene Pks
Protein Family:ABC transporter L-arabinose-binding periplasmic protein
UniProt Gene Name:ARAF  
UniProt Entry Name:ARAF_HUMAN
Anti-ARAF Antibody (CAB16346)Immunofluorescence analysis of HeLa cells using ARAF antibody (CAB16346) at dilution of 1:100. Blue: DAPI for nuclear staining.
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